A prediction of the amino acids and structures involved in DNA recognition by type I DNA restriction and modification enzymes

S S Sturrock, D T F Dryden

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The S subunits of type I DNA restriction/modification enzymes are responsible for recognising the DNA target sequence for the enzyme. They contain two domains of approximately 150 amino acids, each of which is responsible for recognising one half of the bipartite asymmetric target. In the absence of any known tertiary structure for type I enzymes or recognisable DNA recognition motifs in the highly variable amino acid sequences of the S subunits, it has previously not been possible to predict which amino acids are responsible for sequence recognition, Using a combination of sequence alignment the same tertiary structure. Furthermore, this structure is similar to the structure of the TRD of the C5-cytosine methyltransferase, Hhal, which recognises its DNA target via interactions with two short polypeptide loops and a beta strand, Our results predict the location of these sequence recognition structures within the TRDs of all type IS subunits.

Original languageEnglish
Pages (from-to)3408-3414
Number of pages7
JournalNucleic Acids Research
Volume25
Issue number17
DOIs
Publication statusPublished - 1 Sept 1997

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