A rapid protocol for generating arthropod DNA barcodes suitable for use with undergraduate students

Grant R. Brown; Claire Dagen; Ben Reilly O’Donnell; Jeff A. Graves

doi:10.1080/00219266.2019.1620312

A rapid protocol for generating arthropod DNA barcodes suitable for use with undergraduate students

Grant R. Brown, Claire Dagen, Ben Reilly O’Donnell, Jeff A. Graves

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Abstract

We provide a protocol for rapid DNA extraction from spiders suitable for undergraduate practical sessions. Students who were previously naïve to the theory and laboratory technique of DNA barcoding were successfully able to extract and recover 29 DNA sequences from 16 species of small spiders in the family Linyphiidae. We anticipate that with careful selection of specimens, undergraduate students could participate in sessions which both benefit their professional development and further taxonomic understanding across a variety of organisms.

Original language	English
Pages (from-to)	521-529
Number of pages	9
Journal	Journal of Biological Education
Volume	54
Issue number	5
DOIs	https://doi.org/10.1080/00219266.2019.1620312
Publication status	Published - 2020

Keywords / Materials (for Non-textual outputs)

citizen science
Education
interdisciplinary
systematics
taxonomy

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10.1080/00219266.2019.1620312

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title = "A rapid protocol for generating arthropod DNA barcodes suitable for use with undergraduate students",

abstract = "We provide a protocol for rapid DNA extraction from spiders suitable for undergraduate practical sessions. Students who were previously na{\"i}ve to the theory and laboratory technique of DNA barcoding were successfully able to extract and recover 29 DNA sequences from 16 species of small spiders in the family Linyphiidae. We anticipate that with careful selection of specimens, undergraduate students could participate in sessions which both benefit their professional development and further taxonomic understanding across a variety of organisms.",

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AU - Graves, Jeff A.

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Y1 - 2020

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AB - We provide a protocol for rapid DNA extraction from spiders suitable for undergraduate practical sessions. Students who were previously naïve to the theory and laboratory technique of DNA barcoding were successfully able to extract and recover 29 DNA sequences from 16 species of small spiders in the family Linyphiidae. We anticipate that with careful selection of specimens, undergraduate students could participate in sessions which both benefit their professional development and further taxonomic understanding across a variety of organisms.

KW - citizen science

KW - Education

KW - interdisciplinary

KW - systematics

KW - taxonomy

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DO - 10.1080/00219266.2019.1620312

M3 - Article

SN - 0021-9266

VL - 54

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EP - 529

JO - Journal of Biological Education

JF - Journal of Biological Education

IS - 5

ER -

A rapid protocol for generating arthropod DNA barcodes suitable for use with undergraduate students

Abstract

Keywords / Materials (for Non-textual outputs)

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