A scaleable and defined system for generating neural stem cells from human embryonic stem cells

Alexis J. Joannides; Christelle Fiore-Heriche; Alysia A. Battersby; Pandula Athauda-Arachchi; Isabelle A. Bouhon; Lydia Williams; Kristine Westmore; Paul J. Kemp; Alastair Compston; Nicholas D. Allen; Siddharthan Chandran

doi:10.1634/stemcells.2006-0562

A scaleable and defined system for generating neural stem cells from human embryonic stem cells

Alexis J. Joannides, Christelle Fiore-Heriche, Alysia A. Battersby, Pandula Athauda-Arachchi, Isabelle A. Bouhon, Lydia Williams, Kristine Westmore, Paul J. Kemp, Alastair Compston, Nicholas D. Allen, Siddharthan Chandran^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The ability to differentiate human ESCs (hESCs) to defined lineages in a totally controlled manner is fundamental to developing cell-based therapies and studying human developmental mechanisms. We report a novel, scaleable, and widely applicable system for deriving and propagating neural stem cells from hESCs without the use of animal products, proprietary formulations, or genetic manipulation. This system provides a definitive platform for studying human neural development and has potential therapeutic implications.

Original language	English
Pages (from-to)	731-737
Number of pages	7
Journal	STEM CELLS
Volume	25
Issue number	3
DOIs	https://doi.org/10.1634/stemcells.2006-0562
Publication status	Published - Mar 2007

Keywords / Materials (for Non-textual outputs)

human embryonic stem cells
neural stem cells
defined conditions
fibroblast growth factor
HUMAN ES CELLS
DIRECTED DIFFERENTIATION
PARKINSONS-DISEASE
SELF-RENEWAL
IN-VITRO
DOPAMINERGIC-NEURONS
PROGENITOR CELLS
PRECURSOR CELLS
CULTURE
DERIVATION

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10.1634/stemcells.2006-0562

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title = "A scaleable and defined system for generating neural stem cells from human embryonic stem cells",

abstract = "The ability to differentiate human ESCs (hESCs) to defined lineages in a totally controlled manner is fundamental to developing cell-based therapies and studying human developmental mechanisms. We report a novel, scaleable, and widely applicable system for deriving and propagating neural stem cells from hESCs without the use of animal products, proprietary formulations, or genetic manipulation. This system provides a definitive platform for studying human neural development and has potential therapeutic implications.",

keywords = "human embryonic stem cells, neural stem cells, defined conditions, fibroblast growth factor, HUMAN ES CELLS, DIRECTED DIFFERENTIATION, PARKINSONS-DISEASE, SELF-RENEWAL, IN-VITRO, DOPAMINERGIC-NEURONS, PROGENITOR CELLS, PRECURSOR CELLS, CULTURE, DERIVATION",

author = "Joannides, {Alexis J.} and Christelle Fiore-Heriche and Battersby, {Alysia A.} and Pandula Athauda-Arachchi and Bouhon, {Isabelle A.} and Lydia Williams and Kristine Westmore and Kemp, {Paul J.} and Alastair Compston and Allen, {Nicholas D.} and Siddharthan Chandran",

year = "2007",

month = mar,

doi = "10.1634/stemcells.2006-0562",

language = "English",

volume = "25",

pages = "731--737",

journal = "STEM CELLS",

issn = "1066-5099",

publisher = "Wiley-Blackwell",

number = "3",

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TY - JOUR

T1 - A scaleable and defined system for generating neural stem cells from human embryonic stem cells

AU - Joannides, Alexis J.

AU - Fiore-Heriche, Christelle

AU - Battersby, Alysia A.

AU - Athauda-Arachchi, Pandula

AU - Bouhon, Isabelle A.

AU - Williams, Lydia

AU - Westmore, Kristine

AU - Kemp, Paul J.

AU - Compston, Alastair

AU - Allen, Nicholas D.

AU - Chandran, Siddharthan

PY - 2007/3

Y1 - 2007/3

N2 - The ability to differentiate human ESCs (hESCs) to defined lineages in a totally controlled manner is fundamental to developing cell-based therapies and studying human developmental mechanisms. We report a novel, scaleable, and widely applicable system for deriving and propagating neural stem cells from hESCs without the use of animal products, proprietary formulations, or genetic manipulation. This system provides a definitive platform for studying human neural development and has potential therapeutic implications.

AB - The ability to differentiate human ESCs (hESCs) to defined lineages in a totally controlled manner is fundamental to developing cell-based therapies and studying human developmental mechanisms. We report a novel, scaleable, and widely applicable system for deriving and propagating neural stem cells from hESCs without the use of animal products, proprietary formulations, or genetic manipulation. This system provides a definitive platform for studying human neural development and has potential therapeutic implications.

KW - human embryonic stem cells

KW - neural stem cells

KW - defined conditions

KW - fibroblast growth factor

KW - HUMAN ES CELLS

KW - DIRECTED DIFFERENTIATION

KW - PARKINSONS-DISEASE

KW - SELF-RENEWAL

KW - IN-VITRO

KW - DOPAMINERGIC-NEURONS

KW - PROGENITOR CELLS

KW - PRECURSOR CELLS

KW - CULTURE

KW - DERIVATION

U2 - 10.1634/stemcells.2006-0562

DO - 10.1634/stemcells.2006-0562

M3 - Article

SN - 1066-5099

VL - 25

SP - 731

EP - 737

JO - STEM CELLS

JF - STEM CELLS

IS - 3

ER -

A scaleable and defined system for generating neural stem cells from human embryonic stem cells

Abstract

Keywords / Materials (for Non-textual outputs)

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