Projects per year
Abstract
Mapping the molecular composition of individual excitatory synapses across the mouse brain reveals high synapse diversity with each brain region showing a distinct composition of synapse types. As a first step towards systematic mapping of synapse diversity across the human brain, we have labelled and imaged synapses expressing the excitatory synapse protein PSD95 in twenty human brain regions, including 13 neocortical, two subcortical, one hippocampal, one cerebellar and three brainstem regions, in four phenotypically normal individuals. We quantified the number, size and intensity of individual synaptic puncta and compared their regional distributions. We found that each region showed a distinct signature of synaptic puncta parameters. Comparison of brain regions showed that cortical and hippocampal structures are similar, and distinct from those of cerebellum and brainstem. Comparison of synapse parameters from human and mouse brain revealed conservation of parameters, hierarchical organization of brain regions and network architecture. This work illustrates the feasibility of generating a systematic single‐synapse resolution atlas of the human brain, a potentially significant resource in studies of brain health and disease.
Original language | English |
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Journal | European Journal of Neuroscience |
DOIs | |
Publication status | Published - 3 Jun 2020 |
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Dive into the research topics of 'A single‐synapse resolution survey of PSD95‐positive synapses in twenty human brain regions'. Together they form a unique fingerprint.Projects
- 5 Finished
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MRC Brain Banks: Joint Application to Underpin Neuroscience Research
1/11/13 → 31/10/22
Project: Research
Profiles
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Colin Smith
- Deanery of Clinical Sciences - Personal Chair Neuropathology
- Centre for Clinical Brain Sciences
- Euan MacDonald Centre for Motor Neuron Disease Research
- Edinburgh Neuroscience
- Cerebrovascular Research Group
Person: Academic: Research Active