A study on lymphocyte activation in maedi-visna virus induced pneumonia

I Begara; L Lujan; J Hopkins; D D Collie; H R Miller; N J Watt

doi:10.1016/0165-2427(94)05354-U

A study on lymphocyte activation in maedi-visna virus induced pneumonia

I Begara, L Lujan, J Hopkins, D D Collie, H R Miller, N J Watt

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The stage of activation of bronchoalveolar lavage fluid (BALF) lymphocytes and peripheral blood lymphocytes (PBL) from maedi-visna virus (MVV) infected (n = 7) and control (n = 7) sheep was investigated by assessing four parameters of lymphocyte activation; lymphocyte size and complexity, loss of CD5+ T cells, expression of cell surface interleukin-2 receptor (IL-2R) and expression of DR and DQ MHC Class II molecules. BALF lymphocytes from MVV-infected animals had a significant loss of CD5+ lymphocytes (P <0.05) and upregulation of DR and DQ MHC Class II molecules compared with controls, consistent with BALF lymphocyte activation. No changes in cell size and complexity or expression of IL-2R were observed. No evidence of PBL activation was detected. These findings suggest an impaired BALF lymphocyte activation during MVV infection.

Original language	English
Pages (from-to)	197-210
Number of pages	14
Journal	Veterinary Immunology and Immunopathology
Volume	45
Issue number	3-4
DOIs	https://doi.org/10.1016/0165-2427(94)05354-U
Publication status	Published - Apr 1995

Keywords / Materials (for Non-textual outputs)

Animals
Sheep
Visna-maedi virus
Receptors, Interleukin-2
Antibodies, Monoclonal
Lymphocyte Activation
Lymphocyte Count
HLA-DQ Antigens
Pneumonia, Progressive Interstitial, of Sheep
HLA-DR Antigens
Antigens, CD
Antigens, CD5
Bronchoalveolar Lavage Fluid
Flow Cytometry
Up-Regulation
Immunophenotyping
Female
T-Lymphocytes

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10.1016/0165-2427(94)05354-U

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@article{c0819f9f371f4bae8d8b14fd37d1350a,

title = "A study on lymphocyte activation in maedi-visna virus induced pneumonia",

abstract = "The stage of activation of bronchoalveolar lavage fluid (BALF) lymphocytes and peripheral blood lymphocytes (PBL) from maedi-visna virus (MVV) infected (n = 7) and control (n = 7) sheep was investigated by assessing four parameters of lymphocyte activation; lymphocyte size and complexity, loss of CD5+ T cells, expression of cell surface interleukin-2 receptor (IL-2R) and expression of DR and DQ MHC Class II molecules. BALF lymphocytes from MVV-infected animals had a significant loss of CD5+ lymphocytes (P <0.05) and upregulation of DR and DQ MHC Class II molecules compared with controls, consistent with BALF lymphocyte activation. No changes in cell size and complexity or expression of IL-2R were observed. No evidence of PBL activation was detected. These findings suggest an impaired BALF lymphocyte activation during MVV infection.",

keywords = "Animals, Sheep, Visna-maedi virus, Receptors, Interleukin-2, Antibodies, Monoclonal, Lymphocyte Activation, Lymphocyte Count, HLA-DQ Antigens, Pneumonia, Progressive Interstitial, of Sheep, HLA-DR Antigens, Antigens, CD, Antigens, CD5, Bronchoalveolar Lavage Fluid, Flow Cytometry, Up-Regulation, Immunophenotyping, Female, T-Lymphocytes",

author = "I Begara and L Lujan and J Hopkins and Collie, {D D} and Miller, {H R} and Watt, {N J}",

year = "1995",

month = apr,

doi = "10.1016/0165-2427(94)05354-U",

language = "English",

volume = "45",

pages = "197--210",

journal = "Veterinary Immunology and Immunopathology",

issn = "0165-2427",

publisher = "Elsevier",

number = "3-4",

}

TY - JOUR

T1 - A study on lymphocyte activation in maedi-visna virus induced pneumonia

AU - Begara, I

AU - Lujan, L

AU - Hopkins, J

AU - Collie, D D

AU - Miller, H R

AU - Watt, N J

PY - 1995/4

Y1 - 1995/4

N2 - The stage of activation of bronchoalveolar lavage fluid (BALF) lymphocytes and peripheral blood lymphocytes (PBL) from maedi-visna virus (MVV) infected (n = 7) and control (n = 7) sheep was investigated by assessing four parameters of lymphocyte activation; lymphocyte size and complexity, loss of CD5+ T cells, expression of cell surface interleukin-2 receptor (IL-2R) and expression of DR and DQ MHC Class II molecules. BALF lymphocytes from MVV-infected animals had a significant loss of CD5+ lymphocytes (P <0.05) and upregulation of DR and DQ MHC Class II molecules compared with controls, consistent with BALF lymphocyte activation. No changes in cell size and complexity or expression of IL-2R were observed. No evidence of PBL activation was detected. These findings suggest an impaired BALF lymphocyte activation during MVV infection.

AB - The stage of activation of bronchoalveolar lavage fluid (BALF) lymphocytes and peripheral blood lymphocytes (PBL) from maedi-visna virus (MVV) infected (n = 7) and control (n = 7) sheep was investigated by assessing four parameters of lymphocyte activation; lymphocyte size and complexity, loss of CD5+ T cells, expression of cell surface interleukin-2 receptor (IL-2R) and expression of DR and DQ MHC Class II molecules. BALF lymphocytes from MVV-infected animals had a significant loss of CD5+ lymphocytes (P <0.05) and upregulation of DR and DQ MHC Class II molecules compared with controls, consistent with BALF lymphocyte activation. No changes in cell size and complexity or expression of IL-2R were observed. No evidence of PBL activation was detected. These findings suggest an impaired BALF lymphocyte activation during MVV infection.

KW - Animals

KW - Sheep

KW - Visna-maedi virus

KW - Receptors, Interleukin-2

KW - Antibodies, Monoclonal

KW - Lymphocyte Activation

KW - Lymphocyte Count

KW - HLA-DQ Antigens

KW - Pneumonia, Progressive Interstitial, of Sheep

KW - HLA-DR Antigens

KW - Antigens, CD

KW - Antigens, CD5

KW - Bronchoalveolar Lavage Fluid

KW - Flow Cytometry

KW - Up-Regulation

KW - Immunophenotyping

KW - Female

KW - T-Lymphocytes

U2 - 10.1016/0165-2427(94)05354-U

DO - 10.1016/0165-2427(94)05354-U

M3 - Article

C2 - 7545850

SN - 0165-2427

VL - 45

SP - 197

EP - 210

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 3-4

ER -

A study on lymphocyte activation in maedi-visna virus induced pneumonia

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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