A sub-30 mpH resolution thin film transistor-based nanoribbon biosensing platform

Ioannis Zeimpekis; Konstantinos I. Papadimitriou; Kai Sun; Chunxiao Hu; Peter Ashburn; Hywel Morgan; Themistoklis Prodromakis

doi:10.3390/s17092000

A sub-30 mpH resolution thin film transistor-based nanoribbon biosensing platform

Ioannis Zeimpekis, Konstantinos I. Papadimitriou^*, Kai Sun, Chunxiao Hu, Peter Ashburn, Hywel Morgan, Themistoklis Prodromakis

^*Corresponding author for this work

School of Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

We present a complete biosensing system that comprises a Thin Film Transistor (TFT)-based nanoribbon biosensor and a low noise, high-performance bioinstrumentation platform, capable of detecting sub-30 mpH unit changes, validated by an enzymatic biochemical reaction. The nanoribbon biosensor was fabricated top-down with an ultra-thin (15 nm) polysilicon semiconducting channel that offers excellent sensitivity to surface potential changes. The sensor is coupled to an integrated circuit (IC), which combines dual switched-capacitor integrators with high precision analog-to-digital converters (ADCs). Throughout this work, we employed both conventional pH buffer measurements as well as urea-urease enzymatic reactions for benchmarking the overall performance of the system. The measured results from the urea-urease reaction demonstrate that the system can detect urea in concentrations as low as 25 µM, which translates to a change of 27 mpH, according to our initial pH characterisation measurements. The attained accuracy and resolution of our system as well as its low-cost manufacturability, high processing speed and portability make it a competitive solution for applications requiring rapid and accurate results at remote locations; a necessity for Point-of-Care (POC) diagnostic platforms.

Original language	English
Article number	2000
Journal	Sensors (Switzerland)
Volume	17
Issue number	9
DOIs	https://doi.org/10.3390/s17092000
Publication status	Published - 1 Sept 2017

Keywords / Materials (for Non-textual outputs)

Analog-to-digital conversion
Biosensor
Nanoribbon
PH sensing
Point-of-Care diagnostics
Switched capacitor
TFT
Urea-urease reaction

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10.3390/s17092000

https://www.mdpi.com/1424-8220/17/9/2000Licence: Creative Commons: Attribution (CC-BY)

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title = "A sub-30 mpH resolution thin film transistor-based nanoribbon biosensing platform",

abstract = "We present a complete biosensing system that comprises a Thin Film Transistor (TFT)-based nanoribbon biosensor and a low noise, high-performance bioinstrumentation platform, capable of detecting sub-30 mpH unit changes, validated by an enzymatic biochemical reaction. The nanoribbon biosensor was fabricated top-down with an ultra-thin (15 nm) polysilicon semiconducting channel that offers excellent sensitivity to surface potential changes. The sensor is coupled to an integrated circuit (IC), which combines dual switched-capacitor integrators with high precision analog-to-digital converters (ADCs). Throughout this work, we employed both conventional pH buffer measurements as well as urea-urease enzymatic reactions for benchmarking the overall performance of the system. The measured results from the urea-urease reaction demonstrate that the system can detect urea in concentrations as low as 25 µM, which translates to a change of 27 mpH, according to our initial pH characterisation measurements. The attained accuracy and resolution of our system as well as its low-cost manufacturability, high processing speed and portability make it a competitive solution for applications requiring rapid and accurate results at remote locations; a necessity for Point-of-Care (POC) diagnostic platforms.",

keywords = "Analog-to-digital conversion, Biosensor, Nanoribbon, PH sensing, Point-of-Care diagnostics, Switched capacitor, TFT, Urea-urease reaction",

author = "Ioannis Zeimpekis and Papadimitriou, {Konstantinos I.} and Kai Sun and Chunxiao Hu and Peter Ashburn and Hywel Morgan and Themistoklis Prodromakis",

note = "Funding Information: Acknowledgments:Author ContributioThensauthors: I.Z. and K.Iacknowledge.P. conceithevedfinancialthe experisupportments,ofI.Z.the, K.EngineeringI.P. and C.H.andcoPhysicalnducted the Sciences Research Council (EPSRC), EP/L020920/1 research grant. The data from this paper can be obtained from experiments, I.Z., K.I.P., K.S. and C.H. analysed the results, I.Z., K.I.P., K.S., C.H., P.A., H.M. and T.P. wrote the University of Southampton Institutional Repository http://dx.doi.org/10.5258/SOTON/D0223. manuscript. All authors read, reviewed and have given approval to submit the manuscript. Author Contributions: I.Z. and K.I.P. conceived the experiments, I.Z., K.I.P. and C.H. conducted the experiments, I.Z.,KConflicts of Interest:.I.P., K.S. and C.H.Theanalysedauthors decthe rlare noesults,confliI.Z.,ct Kof .I.interest.P., K.S., C.H., P.A., H.M. and T.P. wrote the manuscript. All authors read, reviewed and have given approval to submit the manuscript. Publisher Copyright: {\textcopyright} 2017 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Zeimpekis, Ioannis

AU - Papadimitriou, Konstantinos I.

AU - Sun, Kai

AU - Hu, Chunxiao

AU - Ashburn, Peter

AU - Morgan, Hywel

AU - Prodromakis, Themistoklis

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N2 - We present a complete biosensing system that comprises a Thin Film Transistor (TFT)-based nanoribbon biosensor and a low noise, high-performance bioinstrumentation platform, capable of detecting sub-30 mpH unit changes, validated by an enzymatic biochemical reaction. The nanoribbon biosensor was fabricated top-down with an ultra-thin (15 nm) polysilicon semiconducting channel that offers excellent sensitivity to surface potential changes. The sensor is coupled to an integrated circuit (IC), which combines dual switched-capacitor integrators with high precision analog-to-digital converters (ADCs). Throughout this work, we employed both conventional pH buffer measurements as well as urea-urease enzymatic reactions for benchmarking the overall performance of the system. The measured results from the urea-urease reaction demonstrate that the system can detect urea in concentrations as low as 25 µM, which translates to a change of 27 mpH, according to our initial pH characterisation measurements. The attained accuracy and resolution of our system as well as its low-cost manufacturability, high processing speed and portability make it a competitive solution for applications requiring rapid and accurate results at remote locations; a necessity for Point-of-Care (POC) diagnostic platforms.

AB - We present a complete biosensing system that comprises a Thin Film Transistor (TFT)-based nanoribbon biosensor and a low noise, high-performance bioinstrumentation platform, capable of detecting sub-30 mpH unit changes, validated by an enzymatic biochemical reaction. The nanoribbon biosensor was fabricated top-down with an ultra-thin (15 nm) polysilicon semiconducting channel that offers excellent sensitivity to surface potential changes. The sensor is coupled to an integrated circuit (IC), which combines dual switched-capacitor integrators with high precision analog-to-digital converters (ADCs). Throughout this work, we employed both conventional pH buffer measurements as well as urea-urease enzymatic reactions for benchmarking the overall performance of the system. The measured results from the urea-urease reaction demonstrate that the system can detect urea in concentrations as low as 25 µM, which translates to a change of 27 mpH, according to our initial pH characterisation measurements. The attained accuracy and resolution of our system as well as its low-cost manufacturability, high processing speed and portability make it a competitive solution for applications requiring rapid and accurate results at remote locations; a necessity for Point-of-Care (POC) diagnostic platforms.

KW - Analog-to-digital conversion

KW - Biosensor

KW - Nanoribbon

KW - PH sensing

KW - Point-of-Care diagnostics

KW - Switched capacitor

KW - TFT

KW - Urea-urease reaction

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DO - 10.3390/s17092000

M3 - Article

C2 - 28862645

AN - SCOPUS:85028731439

SN - 1424-8220

VL - 17

JO - Sensors (Switzerland)

JF - Sensors (Switzerland)

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ER -

A sub-30 mpH resolution thin film transistor-based nanoribbon biosensing platform

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Keywords / Materials (for Non-textual outputs)

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