A switchable light-input, light-output system modelled and constructed in yeast

Oksana Sorokina; Anita Kapus; Kata Terecskei; Laura E Dixon; Laszlo Kozma-Bognar; Ferenc Nagy; Andrew J Millar

doi:10.1186/1754-1611-3-15

A switchable light-input, light-output system modelled and constructed in yeast

Oksana Sorokina, Anita Kapus, Kata Terecskei, Laura E Dixon, Laszlo Kozma-Bognar, Ferenc Nagy, Andrew J Millar

Research output: Contribution to journal › Article › peer-review

Abstract

Background
Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter gene, which we detect by real-time imaging of yeast colonies on solid media.

Results
The reversible activation of the phytochrome by red light, and its inactivation by far-red light, is retained. We use this quantitative readout to construct a mathematical model that matches the system's behaviour and predicts the molecular targets for future manipulation.

Conclusion
Our model, methods and materials together constitute a novel system for a eukaryotic host with the potential to convert a dynamic pattern of light input into a predictable gene expression response. This system could be applied for the regulation of genetic networks - both known and synthetic.

Original language	English
Article number	15
Number of pages	16
Journal	Journal of Biological Engineering
Volume	3
Issue number	15
DOIs	https://doi.org/10.1186/1754-1611-3-15
Publication status	Published - Sep 2009

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10.1186/1754-1611-3-15

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http://www.jbioleng.org/content/3/1/15

SynthSys; formerly CSBE: Centre for Systems Biology at Edinburgh
Millar, A., Beggs, J., Ghazal, P., Goryanin, I., Hillston, J., Plotkin, G., Tollervey, D., Walton, A. & Robertson, K.
BBSRC
8/01/07 → 31/12/12
Project: Research
EUCLOCK: Entrainment of the CIrcadian Clock
Millar, A.
EU government bodies
1/01/06 → 30/06/11
Project: Research
Research Development Fellowship:Professor A J Millar - Mathematical and synthetic models of clock gene regulatory circuits.
Millar, A.
BBSRC
15/02/05 → 14/12/07
Project: Research

Cite this

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title = "A switchable light-input, light-output system modelled and constructed in yeast",

abstract = "BackgroundAdvances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter gene, which we detect by real-time imaging of yeast colonies on solid media.ResultsThe reversible activation of the phytochrome by red light, and its inactivation by far-red light, is retained. We use this quantitative readout to construct a mathematical model that matches the system's behaviour and predicts the molecular targets for future manipulation.ConclusionOur model, methods and materials together constitute a novel system for a eukaryotic host with the potential to convert a dynamic pattern of light input into a predictable gene expression response. This system could be applied for the regulation of genetic networks - both known and synthetic.",

author = "Oksana Sorokina and Anita Kapus and Kata Terecskei and Dixon, {Laura E} and Laszlo Kozma-Bognar and Ferenc Nagy and Millar, {Andrew J}",

year = "2009",

month = sep,

doi = "10.1186/1754-1611-3-15",

language = "English",

volume = "3",

journal = "Journal of Biological Engineering",

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number = "15",

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T1 - A switchable light-input, light-output system modelled and constructed in yeast

AU - Sorokina, Oksana

AU - Kapus, Anita

AU - Terecskei, Kata

AU - Dixon, Laura E

AU - Kozma-Bognar, Laszlo

AU - Nagy, Ferenc

AU - Millar, Andrew J

PY - 2009/9

Y1 - 2009/9

N2 - BackgroundAdvances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter gene, which we detect by real-time imaging of yeast colonies on solid media.ResultsThe reversible activation of the phytochrome by red light, and its inactivation by far-red light, is retained. We use this quantitative readout to construct a mathematical model that matches the system's behaviour and predicts the molecular targets for future manipulation.ConclusionOur model, methods and materials together constitute a novel system for a eukaryotic host with the potential to convert a dynamic pattern of light input into a predictable gene expression response. This system could be applied for the regulation of genetic networks - both known and synthetic.

AB - BackgroundAdvances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter gene, which we detect by real-time imaging of yeast colonies on solid media.ResultsThe reversible activation of the phytochrome by red light, and its inactivation by far-red light, is retained. We use this quantitative readout to construct a mathematical model that matches the system's behaviour and predicts the molecular targets for future manipulation.ConclusionOur model, methods and materials together constitute a novel system for a eukaryotic host with the potential to convert a dynamic pattern of light input into a predictable gene expression response. This system could be applied for the regulation of genetic networks - both known and synthetic.

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JO - Journal of Biological Engineering

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A switchable light-input, light-output system modelled and constructed in yeast

Abstract

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Projects

SynthSys; formerly CSBE: Centre for Systems Biology at Edinburgh

EUCLOCK: Entrainment of the CIrcadian Clock

Research Development Fellowship:Professor A J Millar - Mathematical and synthetic models of clock gene regulatory circuits.

Cite this