A tandem enzymatic sp2-C-methylation process: Coupling in situ s-adenosyl-L-methionine formation with methyl transfer

Joanna C. Sadler, Luke D. Humphreys, Radka Snajdrova, Glenn A. Burley

Research output: Contribution to journalArticlepeer-review

Abstract

A one‐pot, two‐step biocatalytic platform for the regiospecfic C‐methylation and C‐ethylation of aromatic substrates is described. The tandem process utilises SalL (Salinospora tropica) for in situ synthesis of S‐adenosyl‐l‐methionine (SAM), followed by alkylation of aromatic substrates by the C‐methyltransferase NovO (Streptomyces spheroides). The application of this methodology is demonstrated for the regiospecific labelling of aromatic substrates by the transfer of methyl, ethyl and isotopically labelled 13CH3, 13CD3 and CD3 groups from their corresponding SAM analogues formed in situ.
Original languageEnglish
Pages (from-to)992-995
JournalChemBioChem
Volume18
Issue number11
DOIs
Publication statusPublished - 1 Jun 2017

Keywords

  • alkylation
  • biocatalysis
  • methyltransferase
  • multi-enzyme reaction
  • S-adenosylmethionine

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