Abstract
The spermatogonial stem cell (SSC) that supports spermatogenesis throughout adult life resides within the GFR 1-expressing A type undifferentiated spermatogonia. The decision to commit to spermatogenic differentiation coincides with the loss of GFR 1 and reciprocal gain of Ngn3 (Neurog3) expression. Through the analysis of the piRNA factor Miwi2 (Piwil4), we identify a novel population of Ngn3-expressing spermatogonia that are essential for efficient testicular regeneration after injury.
Depletion of Miwi2-expressing cells results in a transient impact on testicular
homeostasis with this population behaving strictly as transit amplifying cells under homeostatic conditions. However, upon injury Miwi2-expressing cells are essential for the efficient regenerative capacity of the testis and also display facultative stem activity in transplantation assays. In summary, the mouse testis has adopted a regenerative strategy to expand stem cell activity by incorporating a transit amplifying population to the effective stem cell pool, thus ensuring rapid and efficient tissue repair.
Depletion of Miwi2-expressing cells results in a transient impact on testicular
homeostasis with this population behaving strictly as transit amplifying cells under homeostatic conditions. However, upon injury Miwi2-expressing cells are essential for the efficient regenerative capacity of the testis and also display facultative stem activity in transplantation assays. In summary, the mouse testis has adopted a regenerative strategy to expand stem cell activity by incorporating a transit amplifying population to the effective stem cell pool, thus ensuring rapid and efficient tissue repair.
Original language | English |
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Pages (from-to) | 1631-1641 |
Number of pages | 11 |
Journal | Journal of Experimental Medicine |
Volume | 214 |
Issue number | 6 |
Early online date | 1 May 2017 |
DOIs | |
Publication status | Published - 1 Jun 2017 |
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Donal O'Carroll
- School of Biological Sciences - Chair of Stem Cell Biology
- Centre for Regenerative Medicine
Person: Academic: Research Active