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Abstract
The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species.
Original language | English |
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Pages (from-to) | 1-9 |
Number of pages | 9 |
Journal | PLoS ONE |
Volume | 18 |
Issue number | 9 |
Early online date | 7 Sept 2023 |
DOIs | |
Publication status | Published - Sept 2023 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Cell Nucleus/genetics
- Salmo salar
- Technology
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Dive into the research topics of 'A versatile nuclei extraction protocol for single nucleus sequencing in non-model species – optimization in various Atlantic salmon tissues'. Together they form a unique fingerprint.Projects
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Equipment
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Genomics Platform
James Furniss (Manager)
Royal (Dick) School of Veterinary StudiesFacility/equipment: Facility