A versatile nuclei extraction protocol for single nucleus sequencing in non-model species – optimization in various Atlantic salmon tissues

Rose Ruiz Daniels*, Richard Taylor, Ross Dobie, Sarah Salisbury, James Furniss, Emily Clark, Dan Macqueen, Diego Robledo

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalPLoS ONE
Volume18
Issue number9
Early online date7 Sept 2023
DOIs
Publication statusPublished - Sept 2023

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Cell Nucleus/genetics
  • Salmo salar
  • Technology

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