Abattoir-based estimates of mycobacterial infections in Cameroon

Nkongo Franklyn Egbe; Adrian Muwonge; Lucy Ndip; Robert Kelly; Melissa Sander; Vincent N Tanya; Victor Ngu Ngwa; Ian Handel; Amanda Novak; Richard Ngandalo; Stella Mazeri; Kenton L. Morgan; Anne Asuquo; Mark Bronsvoort

doi:10.1038/srep24320

Abattoir-based estimates of mycobacterial infections in Cameroon

Nkongo Franklyn Egbe, Adrian Muwonge, Lucy Ndip, Robert Kelly, Melissa Sander, Vincent N Tanya, Victor Ngu Ngwa, Ian Handel, Amanda Novak, Richard Ngandalo, Stella Mazeri, Kenton L. Morgan, Anne Asuquo, Mark Bronsvoort

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Mycobacteria cause major diseases including human tuberculosis, bovine tuberculosis and Johne's disease. In livestock, the dominant species is M. bovis causing bovine tuberculosis (bTB), a disease of global zoonotic importance. In this study, we estimated the prevalence of Mycobacteria in slaughter cattle in Cameroon.
A total of 2,346 cattle were examined in a cross-sectional study at four abattoirs in Cameroon. Up to three lesions per animal were collected for further study and a retropharyngeal lymph node was collected from a random sample of non-lesioned animals. Samples were cultured on Lowenstein Jensen media and the BACTEC MGIT 960 system, and identified using the Hain® Genotype kits.
A total of 207/2,346 cattle were identified with bTB-like lesions, representing 4.0% (45/1,129), 11.3% (106/935), 23.8% (38/160) and 14.8% (18/122) of the cattle in the Bamenda, Ngaoundere, Garoua and Maroua abattoirs respectively. The minimum estimated prevalence of M. bovis was 2.8% (1.9-3.9), 7.7% (6.1-9.6), 21.3% (15.2-28.4) and 13.1% (7.7-20.4) in the four abattoirs respectively. One M. tuberculosis and three M. bovis strains were recovered from non-lesioned animals.
The high prevalence of M. bovis is of public health concern and limits the potential control options in this setting without a viable vaccine as an alternative.

Original language	English
Article number	24320
Journal	Scientific Reports
Volume	6
Early online date	14 Apr 2016
DOIs	https://doi.org/10.1038/srep24320
Publication status	E-pub ahead of print - 14 Apr 2016

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10.1038/srep24320

Abattoir-based estimates of mycobacterial infections in Cameroon
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http://www.nature.com/articles/srep24320

CAMBTB: The phylogenetics and epidemiology of tuberculosis in cattle and their herders in cameroon
Bronsvoort, M. (Principal Investigator)
UK-based charities
1/11/12 → 30/04/14
Project: Research

Dr Adrian Muwonge comments on a proposed global roadmap for fighting zoonotic tuberculosis
Muwonge, A.
11/05/16
1 item of Media coverage
Press/Media: Expert Comment

Mark Bronsvoort
- mark.bronsvoortroslin.ed.acuk
- Royal (Dick) School of Veterinary Studies - Personal Chair of Veterinary Epidemiology
Person: Academic: Research Active
Rob Kelly
- Royal (Dick) School of Veterinary Studies - Lecturer in Veterinary Parasitology
- Global Academy of Agriculture and Food Systems
Person: Academic: Research Active , Academic: Research Active (Teaching)
Adrian Muwonge
- adrian.muwongeroslin.ed.acuk
- Royal (Dick) School of Veterinary Studies - Senior Research Fellow
Person: Academic: Research Active (Research Assistant)

Cite this

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title = "Abattoir-based estimates of mycobacterial infections in Cameroon",

abstract = "Mycobacteria cause major diseases including human tuberculosis, bovine tuberculosis and Johne's disease. In livestock, the dominant species is M. bovis causing bovine tuberculosis (bTB), a disease of global zoonotic importance. In this study, we estimated the prevalence of Mycobacteria in slaughter cattle in Cameroon.A total of 2,346 cattle were examined in a cross-sectional study at four abattoirs in Cameroon. Up to three lesions per animal were collected for further study and a retropharyngeal lymph node was collected from a random sample of non-lesioned animals. Samples were cultured on Lowenstein Jensen media and the BACTEC MGIT 960 system, and identified using the Hain{\textregistered} Genotype kits.A total of 207/2,346 cattle were identified with bTB-like lesions, representing 4.0% (45/1,129), 11.3% (106/935), 23.8% (38/160) and 14.8% (18/122) of the cattle in the Bamenda, Ngaoundere, Garoua and Maroua abattoirs respectively. The minimum estimated prevalence of M. bovis was 2.8% (1.9-3.9), 7.7% (6.1-9.6), 21.3% (15.2-28.4) and 13.1% (7.7-20.4) in the four abattoirs respectively. One M. tuberculosis and three M. bovis strains were recovered from non-lesioned animals. The high prevalence of M. bovis is of public health concern and limits the potential control options in this setting without a viable vaccine as an alternative.",

author = "Egbe, {Nkongo Franklyn} and Adrian Muwonge and Lucy Ndip and Robert Kelly and Melissa Sander and Tanya, {Vincent N} and Ngwa, {Victor Ngu} and Ian Handel and Amanda Novak and Richard Ngandalo and Stella Mazeri and Morgan, {Kenton L.} and Anne Asuquo and Mark Bronsvoort",

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AU - Egbe, Nkongo Franklyn

AU - Muwonge, Adrian

AU - Ndip, Lucy

AU - Kelly, Robert

AU - Sander, Melissa

AU - Tanya, Vincent N

AU - Ngwa, Victor Ngu

AU - Handel, Ian

AU - Novak, Amanda

AU - Ngandalo, Richard

AU - Mazeri, Stella

AU - Morgan, Kenton L.

AU - Asuquo, Anne

AU - Bronsvoort, Mark

PY - 2016/4/14

Y1 - 2016/4/14

N2 - Mycobacteria cause major diseases including human tuberculosis, bovine tuberculosis and Johne's disease. In livestock, the dominant species is M. bovis causing bovine tuberculosis (bTB), a disease of global zoonotic importance. In this study, we estimated the prevalence of Mycobacteria in slaughter cattle in Cameroon.A total of 2,346 cattle were examined in a cross-sectional study at four abattoirs in Cameroon. Up to three lesions per animal were collected for further study and a retropharyngeal lymph node was collected from a random sample of non-lesioned animals. Samples were cultured on Lowenstein Jensen media and the BACTEC MGIT 960 system, and identified using the Hain® Genotype kits.A total of 207/2,346 cattle were identified with bTB-like lesions, representing 4.0% (45/1,129), 11.3% (106/935), 23.8% (38/160) and 14.8% (18/122) of the cattle in the Bamenda, Ngaoundere, Garoua and Maroua abattoirs respectively. The minimum estimated prevalence of M. bovis was 2.8% (1.9-3.9), 7.7% (6.1-9.6), 21.3% (15.2-28.4) and 13.1% (7.7-20.4) in the four abattoirs respectively. One M. tuberculosis and three M. bovis strains were recovered from non-lesioned animals. The high prevalence of M. bovis is of public health concern and limits the potential control options in this setting without a viable vaccine as an alternative.

AB - Mycobacteria cause major diseases including human tuberculosis, bovine tuberculosis and Johne's disease. In livestock, the dominant species is M. bovis causing bovine tuberculosis (bTB), a disease of global zoonotic importance. In this study, we estimated the prevalence of Mycobacteria in slaughter cattle in Cameroon.A total of 2,346 cattle were examined in a cross-sectional study at four abattoirs in Cameroon. Up to three lesions per animal were collected for further study and a retropharyngeal lymph node was collected from a random sample of non-lesioned animals. Samples were cultured on Lowenstein Jensen media and the BACTEC MGIT 960 system, and identified using the Hain® Genotype kits.A total of 207/2,346 cattle were identified with bTB-like lesions, representing 4.0% (45/1,129), 11.3% (106/935), 23.8% (38/160) and 14.8% (18/122) of the cattle in the Bamenda, Ngaoundere, Garoua and Maroua abattoirs respectively. The minimum estimated prevalence of M. bovis was 2.8% (1.9-3.9), 7.7% (6.1-9.6), 21.3% (15.2-28.4) and 13.1% (7.7-20.4) in the four abattoirs respectively. One M. tuberculosis and three M. bovis strains were recovered from non-lesioned animals. The high prevalence of M. bovis is of public health concern and limits the potential control options in this setting without a viable vaccine as an alternative.

U2 - 10.1038/srep24320

DO - 10.1038/srep24320

M3 - Article

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 24320

ER -

Abattoir-based estimates of mycobacterial infections in Cameroon

Abstract

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Projects

CAMBTB: The phylogenetics and epidemiology of tuberculosis in cattle and their herders in cameroon

Press/Media

Dr Adrian Muwonge comments on a proposed global roadmap for fighting zoonotic tuberculosis

Profiles

Mark Bronsvoort

Rob Kelly

Adrian Muwonge

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