Actinobacillus pleuropneumoniae does not require urease activity to produce acute swine pleuropneumonia

Ruben-Ivan Tascón Cabrero; Jose A Vazquez-Boland; Cesar B Gutierrez; J Ignacio Rodriguez-Barbosa; Elias Fernando Rodriguez-Ferri

doi:10.1111/j.1574-6968.1997.tb10266.x

Actinobacillus pleuropneumoniae does not require urease activity to produce acute swine pleuropneumonia

Ruben-Ivan Tascón Cabrero, Jose A Vazquez-Boland, Cesar B Gutierrez, J Ignacio Rodriguez-Barbosa, Elias Fernando Rodriguez-Ferri

Deanery of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The role in virulence of Actinobacillus pleuropneumoniae urease activity was investigated. A urease-negative mutant was isolated following transposon mutagenesis with a mini-Tn10 derivative. Both the parent strain and the urease-negative mutant exhibited identical LD50 values in a murine infection model. Pig challenge confirmed that the urease-negative mutant was fully virulent, since experimental inoculation with 5 x 10(7) colony forming units resulted in an acute disease indistinguishable from that produced by the wild-type strain at the same dose. Our results demonstrate that urease activity is not required for the development of acute pleuropneumonia.

Original language	English
Pages (from-to)	53-7
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	148
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1997.tb10266.x
Publication status	Published - Mar 1997

Keywords / Materials (for Non-textual outputs)

Actinobacillus pleuropneumoniae/enzymology
Actinobacillus pleuropneumoniae/genetics
Actinobacillus pleuropneumoniae/pathogenicity
Animals
Bacterial Proteins/metabolism
Blotting, Southern
DNA Transposable Elements
DNA, Bacterial/analysis
Female
Male
Mice
Mice, Inbred Strains
Mutagenesis
Pleuropneumonia/microbiology
Swine
Swine Diseases/microbiology
Urease/genetics
Urease/metabolism
Virulence

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10.1111/j.1574-6968.1997.tb10266.x

Actinobacillus pleuropneumoniae does not require urease activity to produce acute swine pleuropneumonia
Copyright 1997 Federation of European Microbiological Societies
Final published version, 393 KB

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title = "Actinobacillus pleuropneumoniae does not require urease activity to produce acute swine pleuropneumonia",

abstract = "The role in virulence of Actinobacillus pleuropneumoniae urease activity was investigated. A urease-negative mutant was isolated following transposon mutagenesis with a mini-Tn10 derivative. Both the parent strain and the urease-negative mutant exhibited identical LD50 values in a murine infection model. Pig challenge confirmed that the urease-negative mutant was fully virulent, since experimental inoculation with 5 x 10(7) colony forming units resulted in an acute disease indistinguishable from that produced by the wild-type strain at the same dose. Our results demonstrate that urease activity is not required for the development of acute pleuropneumonia.",

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author = "{Tasc{\'o}n Cabrero}, Ruben-Ivan and Vazquez-Boland, {Jose A} and Gutierrez, {Cesar B} and Rodriguez-Barbosa, {J Ignacio} and Rodriguez-Ferri, {Elias Fernando}",

year = "1997",

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AU - Tascón Cabrero, Ruben-Ivan

AU - Vazquez-Boland, Jose A

AU - Gutierrez, Cesar B

AU - Rodriguez-Barbosa, J Ignacio

AU - Rodriguez-Ferri, Elias Fernando

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N2 - The role in virulence of Actinobacillus pleuropneumoniae urease activity was investigated. A urease-negative mutant was isolated following transposon mutagenesis with a mini-Tn10 derivative. Both the parent strain and the urease-negative mutant exhibited identical LD50 values in a murine infection model. Pig challenge confirmed that the urease-negative mutant was fully virulent, since experimental inoculation with 5 x 10(7) colony forming units resulted in an acute disease indistinguishable from that produced by the wild-type strain at the same dose. Our results demonstrate that urease activity is not required for the development of acute pleuropneumonia.

AB - The role in virulence of Actinobacillus pleuropneumoniae urease activity was investigated. A urease-negative mutant was isolated following transposon mutagenesis with a mini-Tn10 derivative. Both the parent strain and the urease-negative mutant exhibited identical LD50 values in a murine infection model. Pig challenge confirmed that the urease-negative mutant was fully virulent, since experimental inoculation with 5 x 10(7) colony forming units resulted in an acute disease indistinguishable from that produced by the wild-type strain at the same dose. Our results demonstrate that urease activity is not required for the development of acute pleuropneumonia.

KW - Actinobacillus pleuropneumoniae/enzymology

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KW - Bacterial Proteins/metabolism

KW - Blotting, Southern

KW - DNA Transposable Elements

KW - DNA, Bacterial/analysis

KW - Female

KW - Male

KW - Mice

KW - Mice, Inbred Strains

KW - Mutagenesis

KW - Pleuropneumonia/microbiology

KW - Swine

KW - Swine Diseases/microbiology

KW - Urease/genetics

KW - Urease/metabolism

KW - Virulence

U2 - 10.1111/j.1574-6968.1997.tb10266.x

DO - 10.1111/j.1574-6968.1997.tb10266.x

M3 - Article

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JF - FEMS Microbiology Letters

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ER -

Actinobacillus pleuropneumoniae does not require urease activity to produce acute swine pleuropneumonia

Abstract

Keywords / Materials (for Non-textual outputs)

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