Actinobacillus pleuropneumoniae does not require urease activity to produce acute swine pleuropneumonia

Ruben-Ivan Tascón Cabrero, Jose A Vazquez-Boland, Cesar B Gutierrez, J Ignacio Rodriguez-Barbosa, Elias Fernando Rodriguez-Ferri

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The role in virulence of Actinobacillus pleuropneumoniae urease activity was investigated. A urease-negative mutant was isolated following transposon mutagenesis with a mini-Tn10 derivative. Both the parent strain and the urease-negative mutant exhibited identical LD50 values in a murine infection model. Pig challenge confirmed that the urease-negative mutant was fully virulent, since experimental inoculation with 5 x 10(7) colony forming units resulted in an acute disease indistinguishable from that produced by the wild-type strain at the same dose. Our results demonstrate that urease activity is not required for the development of acute pleuropneumonia.
Original languageEnglish
Pages (from-to)53-7
Number of pages5
JournalFEMS Microbiology Letters
Issue number1
Publication statusPublished - Mar 1997

Keywords / Materials (for Non-textual outputs)

  • Actinobacillus pleuropneumoniae/enzymology
  • Actinobacillus pleuropneumoniae/genetics
  • Actinobacillus pleuropneumoniae/pathogenicity
  • Animals
  • Bacterial Proteins/metabolism
  • Blotting, Southern
  • DNA Transposable Elements
  • DNA, Bacterial/analysis
  • Female
  • Male
  • Mice
  • Mice, Inbred Strains
  • Mutagenesis
  • Pleuropneumonia/microbiology
  • Swine
  • Swine Diseases/microbiology
  • Urease/genetics
  • Urease/metabolism
  • Virulence


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