Projects per year
Abstract
The germinal center (GC) reaction in Peyer´s patches (PP) requires continued access to antigens, but how this is achieved is not known. Here we show that activated antigen-specific CCR6+CCR1+GL7- B cells make close contact with M cells in the subepithelial dome (SED). Using in situ photoactivation analysis of antigen-specific SED B cells, we find migration of cells towards the GC. Following antigen injection into ligated intestinal loops containing PPs, 40 % of antigen-specific SEDB cells bind antigen within 2 hours, whereas unspecifc cells do not, indicating B cell-receptor involvment. Antigen-loading is not observed in M cell
deficient mice, but is unperturbed in mice depleted of classical dendritic cells (DC). Thus, we report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transport pathway has a critical role in gut IgA responses, and should be taken into account when developing mucosal vaccines
deficient mice, but is unperturbed in mice depleted of classical dendritic cells (DC). Thus, we report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transport pathway has a critical role in gut IgA responses, and should be taken into account when developing mucosal vaccines
Original language | English |
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Article number | 2423 (2019) |
Pages (from-to) | 1-15 |
Number of pages | 15 |
Journal | Nature Communications |
Volume | 10 |
DOIs | |
Publication status | Published - 3 Jun 2019 |
Keywords
- Antibodies
- Antigen processing and presentation
- B cells
- Mucosal immunology
Fingerprint
Dive into the research topics of 'Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome'. Together they form a unique fingerprint.Projects
- 1 Finished
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Determining the effects of ageing on the innate mucosal immune system
1/11/15 → 31/10/18
Project: Research
Profiles
-
Neil Mabbott
- Royal (Dick) School of Veterinary Studies - Personal Chair of Immunopathology
- Edinburgh Neuroscience
- Edinburgh Imaging
Person: Academic: Research Active