Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome

Rathan Joy Komban; Anneli Stromberg; Adi  Biram; Jakob Cervin; Cristina Lebrero-Fernandez; Neil Mabbott; U Yrlid; Ziv Shulman; Mats Bemark; Nils Lycke

doi:10.1038/s41467-019-10144-w

Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome

Rathan Joy Komban, Anneli Stromberg, Adi Biram , Jakob Cervin, Cristina Lebrero-Fernandez, Neil Mabbott, U Yrlid, Ziv Shulman, Mats Bemark, Nils Lycke

Research output: Contribution to journal › Article › peer-review

Abstract

The germinal center (GC) reaction in Peyer´s patches (PP) requires continued access to antigens, but how this is achieved is not known. Here we show that activated antigen-specific CCR6+CCR1+GL7- B cells make close contact with M cells in the subepithelial dome (SED). Using in situ photoactivation analysis of antigen-specific SED B cells, we find migration of cells towards the GC. Following antigen injection into ligated intestinal loops containing PPs, 40 % of antigen-specific SEDB cells bind antigen within 2 hours, whereas unspecifc cells do not, indicating B cell-receptor involvment. Antigen-loading is not observed in M cell
deficient mice, but is unperturbed in mice depleted of classical dendritic cells (DC). Thus, we report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transport pathway has a critical role in gut IgA responses, and should be taken into account when developing mucosal vaccines

Original language	English
Article number	2423 (2019)
Pages (from-to)	1-15
Number of pages	15
Journal	Nature Communications
Volume	10
DOIs	https://doi.org/10.1038/s41467-019-10144-w
Publication status	Published - 3 Jun 2019

Keywords

Antibodies
Antigen processing and presentation
B cells
Mucosal immunology

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10.1038/s41467-019-10144-w

Activated Peyer′s patch B cells ...
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1 Finished

Determining the effects of ageing on the innate mucosal immune system
Mabbott, N.
BBSRC
1/11/15 → 31/10/18
Project: Research

Neil Mabbott
- neil.mabbottroslin.ed.acuk
- Royal (Dick) School of Veterinary Studies - Personal Chair of Immunopathology
- Edinburgh Neuroscience
- Edinburgh Imaging
Person: Academic: Research Active

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title = "Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome",

abstract = "The germinal center (GC) reaction in Peyer´s patches (PP) requires continued access to antigens, but how this is achieved is not known. Here we show that activated antigen-specific CCR6+CCR1+GL7- B cells make close contact with M cells in the subepithelial dome (SED). Using in situ photoactivation analysis of antigen-specific SED B cells, we find migration of cells towards the GC. Following antigen injection into ligated intestinal loops containing PPs, 40 % of antigen-specific SEDB cells bind antigen within 2 hours, whereas unspecifc cells do not, indicating B cell-receptor involvment. Antigen-loading is not observed in M celldeficient mice, but is unperturbed in mice depleted of classical dendritic cells (DC). Thus, we report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transport pathway has a critical role in gut IgA responses, and should be taken into account when developing mucosal vaccines",

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Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome. / Komban, Rathan Joy; Stromberg, Anneli; Biram , Adi ; Cervin, Jakob; Lebrero-Fernandez, Cristina; Mabbott, Neil; Yrlid, U; Shulman, Ziv; Bemark, Mats; Lycke, Nils.

In: Nature Communications, Vol. 10, 2423 (2019), 03.06.2019, p. 1-15.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome

AU - Komban, Rathan Joy

AU - Stromberg, Anneli

AU - Biram , Adi

AU - Cervin, Jakob

AU - Lebrero-Fernandez, Cristina

AU - Mabbott, Neil

AU - Yrlid, U

AU - Shulman, Ziv

AU - Bemark, Mats

AU - Lycke, Nils

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AB - The germinal center (GC) reaction in Peyer´s patches (PP) requires continued access to antigens, but how this is achieved is not known. Here we show that activated antigen-specific CCR6+CCR1+GL7- B cells make close contact with M cells in the subepithelial dome (SED). Using in situ photoactivation analysis of antigen-specific SED B cells, we find migration of cells towards the GC. Following antigen injection into ligated intestinal loops containing PPs, 40 % of antigen-specific SEDB cells bind antigen within 2 hours, whereas unspecifc cells do not, indicating B cell-receptor involvment. Antigen-loading is not observed in M celldeficient mice, but is unperturbed in mice depleted of classical dendritic cells (DC). Thus, we report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transport pathway has a critical role in gut IgA responses, and should be taken into account when developing mucosal vaccines

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KW - Antigen processing and presentation

KW - B cells

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JF - Nature Communications

SN - 2041-1723

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ER -

Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome

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Determining the effects of ageing on the innate mucosal immune system

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Neil Mabbott

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