Activation of mGluR5 induces rapid and long-lasting protein kinase D phosphorylation in hippocampal neurons

Dilja D Krueger, Emily K Osterweil, Mark F Bear

Research output: Contribution to journalArticlepeer-review

Abstract

Metabotropic glutamate receptors (mGluRs), including mGluR5, play a central role in regulating the strength and plasticity of synaptic connections in the brain. However, the signaling pathways that connect mGluRs to their downstream effectors are not yet fully understood. Here, we report that stimulation of mGluR5 in hippocampal cultures and slices results in phosphorylation of protein kinase D (PKD) at the autophosphorylation site Ser-916. This phosphorylation event occurs within 30 s of stimulation, persists for at least 24 h, and is dependent on activation of phospholipase C and protein kinase C. Our data suggest that activation of PKD may represent a novel signaling pathway linking mGluR5 to its downstream targets. These findings have important implications for the study of the molecular mechanisms underlying mGluR-dependent synaptic plasticity.
Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalJournal of Molecular Neuroscience
Volume42
Issue number1
DOIs
Publication statusPublished - Sep 2010

Keywords

  • Animals
  • Cells, Cultured
  • Enzyme Inhibitors
  • Extracellular Signal-Regulated MAP Kinases
  • Female
  • Hippocampus
  • Male
  • Methoxyhydroxyphenylglycol
  • Mice
  • Mice, Inbred C57BL
  • Neurons
  • Phosphorylation
  • Pregnancy
  • Protein Kinase C
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Ionotropic Glutamate
  • Receptors, Metabotropic Glutamate
  • Signal Transduction
  • Synapses
  • Type C Phospholipases
  • p38 Mitogen-Activated Protein Kinases

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