Acute Loss of Cited2 Impairs Nanog Expression and Decreases Self-Renewal of Mouse Embryonic Stem Cells

Kamil R. Kranc; Daniel V. Oliveira; Alejandro Armesilla-Diaz; Ivette Pacheco-Leyva; Ana Catarina Matias; Ana Luisa Escapa; Chithra Subramani; Helen Wheadon; Marlene Trindade; Jennifer Nichols; Keisuke Kaji; Tariq Enver; Jose Braganca

doi:10.1002/stem.1889

Acute Loss of Cited2 Impairs Nanog Expression and Decreases Self-Renewal of Mouse Embryonic Stem Cells

Kamil R. Kranc, Daniel V. Oliveira, Alejandro Armesilla-Diaz, Ivette Pacheco-Leyva, Ana Catarina Matias, Ana Luisa Escapa, Chithra Subramani, Helen Wheadon, Marlene Trindade, Jennifer Nichols, Keisuke Kaji, Tariq Enver, Jose Braganca^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Identifying novel players of the pluripotency gene regulatory network centered on Oct4, Sox2, and Nanog as well as delineating the interactions within the complex network is key to understanding self-renewal and early cell fate commitment of embryonic stem cells (ESC). While overexpression of the transcriptional regulator Cited2 sustains ESC pluripotency, its role in ESC functions remains unclear. Here, we show that Cited2 is important for proliferation, survival, and self-renewal of mouse ESC. We position Cited2 within the pluripotency gene regulatory network by defining Nanog, Tbx3, and Klf4 as its direct targets. We also demonstrate that the defects caused by Cited2 depletion are, at least in part, rescued by Nanog constitutive expression. Finally, we demonstrate that Cited2 is required for and enhances reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells.

Original language	English
Pages (from-to)	699-712
Number of pages	14
Journal	STEM CELLS
Volume	33
Issue number	3
Early online date	6 Nov 2014
DOIs	https://doi.org/10.1002/stem.1889
Publication status	Published - Mar 2015

Keywords / Materials (for Non-textual outputs)

Transcriptional regulation
Self-renewal
Pluripotency
Cited2
Nanog
TRANSCRIPTIONAL NETWORK
SIGNALING PATHWAYS
HEART DEVELOPMENT
GROUND-STATE
PLURIPOTENCY
COACTIVATOR
GENE
HETEROGENEITY
HIF-1-ALPHA
DEFICIENCY

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10.1002/stem.1889

Kranc_et_al-2015-STEM_CELLS
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Final published version, 13.1 MBLicence: Creative Commons: Attribution (CC-BY)

Keisuke Kaji
- Deanery of Clinical Sciences - Personal Chair of Biology of Reprogramming
- Centre for Regenerative Medicine
Person: Academic: Research Active

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@article{4c6db87727cf49de85899fc7fcb459c3,

title = "Acute Loss of Cited2 Impairs Nanog Expression and Decreases Self-Renewal of Mouse Embryonic Stem Cells",

abstract = "Identifying novel players of the pluripotency gene regulatory network centered on Oct4, Sox2, and Nanog as well as delineating the interactions within the complex network is key to understanding self-renewal and early cell fate commitment of embryonic stem cells (ESC). While overexpression of the transcriptional regulator Cited2 sustains ESC pluripotency, its role in ESC functions remains unclear. Here, we show that Cited2 is important for proliferation, survival, and self-renewal of mouse ESC. We position Cited2 within the pluripotency gene regulatory network by defining Nanog, Tbx3, and Klf4 as its direct targets. We also demonstrate that the defects caused by Cited2 depletion are, at least in part, rescued by Nanog constitutive expression. Finally, we demonstrate that Cited2 is required for and enhances reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells.",

keywords = "Transcriptional regulation, Self-renewal, Pluripotency, Cited2, Nanog, TRANSCRIPTIONAL NETWORK, SIGNALING PATHWAYS, HEART DEVELOPMENT, GROUND-STATE, PLURIPOTENCY, COACTIVATOR, GENE, HETEROGENEITY, HIF-1-ALPHA, DEFICIENCY",

author = "Kranc, {Kamil R.} and Oliveira, {Daniel V.} and Alejandro Armesilla-Diaz and Ivette Pacheco-Leyva and Matias, {Ana Catarina} and Escapa, {Ana Luisa} and Chithra Subramani and Helen Wheadon and Marlene Trindade and Jennifer Nichols and Keisuke Kaji and Tariq Enver and Jose Braganca",

note = "Date of Acceptance: 11/10/2014",

year = "2015",

month = mar,

doi = "10.1002/stem.1889",

language = "English",

volume = "33",

pages = "699--712",

journal = "STEM CELLS",

issn = "1066-5099",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Acute Loss of Cited2 Impairs Nanog Expression and Decreases Self-Renewal of Mouse Embryonic Stem Cells

AU - Kranc, Kamil R.

AU - Oliveira, Daniel V.

AU - Armesilla-Diaz, Alejandro

AU - Pacheco-Leyva, Ivette

AU - Matias, Ana Catarina

AU - Escapa, Ana Luisa

AU - Subramani, Chithra

AU - Wheadon, Helen

AU - Trindade, Marlene

AU - Nichols, Jennifer

AU - Kaji, Keisuke

AU - Enver, Tariq

AU - Braganca, Jose

N1 - Date of Acceptance: 11/10/2014

PY - 2015/3

Y1 - 2015/3

N2 - Identifying novel players of the pluripotency gene regulatory network centered on Oct4, Sox2, and Nanog as well as delineating the interactions within the complex network is key to understanding self-renewal and early cell fate commitment of embryonic stem cells (ESC). While overexpression of the transcriptional regulator Cited2 sustains ESC pluripotency, its role in ESC functions remains unclear. Here, we show that Cited2 is important for proliferation, survival, and self-renewal of mouse ESC. We position Cited2 within the pluripotency gene regulatory network by defining Nanog, Tbx3, and Klf4 as its direct targets. We also demonstrate that the defects caused by Cited2 depletion are, at least in part, rescued by Nanog constitutive expression. Finally, we demonstrate that Cited2 is required for and enhances reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells.

AB - Identifying novel players of the pluripotency gene regulatory network centered on Oct4, Sox2, and Nanog as well as delineating the interactions within the complex network is key to understanding self-renewal and early cell fate commitment of embryonic stem cells (ESC). While overexpression of the transcriptional regulator Cited2 sustains ESC pluripotency, its role in ESC functions remains unclear. Here, we show that Cited2 is important for proliferation, survival, and self-renewal of mouse ESC. We position Cited2 within the pluripotency gene regulatory network by defining Nanog, Tbx3, and Klf4 as its direct targets. We also demonstrate that the defects caused by Cited2 depletion are, at least in part, rescued by Nanog constitutive expression. Finally, we demonstrate that Cited2 is required for and enhances reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells.

KW - Transcriptional regulation

KW - Self-renewal

KW - Pluripotency

KW - Cited2

KW - Nanog

KW - TRANSCRIPTIONAL NETWORK

KW - SIGNALING PATHWAYS

KW - HEART DEVELOPMENT

KW - GROUND-STATE

KW - PLURIPOTENCY

KW - COACTIVATOR

KW - GENE

KW - HETEROGENEITY

KW - HIF-1-ALPHA

KW - DEFICIENCY

U2 - 10.1002/stem.1889

DO - 10.1002/stem.1889

M3 - Article

SN - 1066-5099

VL - 33

SP - 699

EP - 712

JO - STEM CELLS

JF - STEM CELLS

IS - 3

ER -

Acute Loss of Cited2 Impairs Nanog Expression and Decreases Self-Renewal of Mouse Embryonic Stem Cells

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Keywords / Materials (for Non-textual outputs)

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Keisuke Kaji

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