Projects per year
Abstract
The F4/80 antigen, encoded by the Adgre1 locus, has been widely-used as a monocyte-macrophage marker in mice, but its value as a macrophage marker in other species is unclear, and has even been questioned. ADGRE1 is a seven transmembrane G protein-coupled receptor with an extracellular domain containing repeated Epidermal Growth Factor (EGF)-like calcium binding domains. Using a new monoclonal antibody, we demonstrated that ADGRE1 is a myeloid differentiation marker in pigs, absent from progenitors in bone marrow, highly-expressed in mature granulocytes, monocytes, and tissue macrophages and induced by macrophage colony-stimulating factor (CSF1) treatment in vivo. Based upon these observations, we utilized RNA-Seq to assess the expression of ADGRE1 mRNA in bone marrow or monocyte-derived macrophages (MDM) and alveolar macrophages from 8 mammalian species including pig, human, rat, sheep, goat, cow, water buffalo, and horse. ADGRE1 mRNA was expressed by macrophages in each species, with inter-species variation both in expression level and response to lipopolysaccharide (LPS) stimulation. Analysis of the RNA-Seq data also revealed additional exons in several species compared to current Ensembl annotations. The ruminant species and horses appear to encode a complete duplication of the 7 EGF-like domains. In every species, Sashimi plots revealed evidence of exon skipping of the EGF-like domains, which are highly-variable between species and polymorphic in humans. Consistent with these expression patterns, key elements of the promoter and a putative enhancer are also conserved across all species. The rapid evolution of this molecule and related ADGRE family members suggests immune selection and a role in pathogen recognition.
Original language | English |
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Article number | 2246 |
Number of pages | 14 |
Journal | Frontiers in Immunology |
Volume | 9 |
DOIs | |
Publication status | Published - 1 Oct 2018 |
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Dive into the research topics of 'ADGRE1 (EMR1, F4/80) Is a Rapidly-Evolving Gene Expressed in Mammalian Monocyte-Macrophages'. Together they form a unique fingerprint.Projects
- 4 Finished
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ISP 1 2017/22 Blueprints for Healthy Animals
Watson, M. (Principal Investigator)
1/04/17 → 31/03/22
Project: Research
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ISP 2 2017/22 Control of Infectious Diseases
Gally, D. (Principal Investigator)
1/04/17 → 31/03/22
Project: Research
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Transcriptome analysis in Indian buffalo and the genetics of innate immunity
Hume, D. (Principal Investigator), Archibald, A. (Co-investigator) & Bishop, S. (Co-investigator)
1/02/14 → 31/01/18
Project: Research
Profiles
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Jayne Hope
- Royal (Dick) School of Veterinary Studies - Personal Chair of Immunology
- Edinburgh Imaging
Person: Academic: Research Active