ADS1 encodes a MATE-transporter that negatively regulates plant disease resistance

Xinli Sun, Eleanor M. Gilroy, Andrea Chini, Pedro L. Nurmberg, Ingo Hein, Christophe Lacomme, Paul R. J. Birch, Adil Hussain, Byung-Wook Yun, Gary J. Loake

Research output: Contribution to journalArticlepeer-review

Abstract

Multidrug and toxic compound extrusion (MATE) proteins comprise the most recently identified family of multidrug transporters. In plants, the numbers of MATE proteins has undergone a remarkable expansion, underscoring the importance of these transporters within this kingdom.

Here, we describe the identification and characterization of Activated Disease Susceptibility 1 (ADS1) which encodes a putative MATE transport protein. An activation tagging screen uncovered the ads1-Dominant (ads1-D) mutant, which was subsequently characterized by molecular, genetic and biochemical approaches.

The ads1-D mutant was compromised in both basal and nonhost resistance against microbial pathogens. Further, plant defence responses conferred by RPS4 were also disabled in ads1-D plants. By contrast, depletion of ADS1 transcripts by RNA-interference (RNAi) promoted basal disease resistance. Unexpectedly, ads1-D plants were found to constitutively accumulate reactive oxygen intermediates (ROIs). However, analysis of ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants indicated that an increase in ROIs did not impact ads1-D-mediated disease susceptibility.

Our findings imply that ADS1 negatively regulates the accumulation of the plant immune activator salicylic acid (SA) and cognate Pathogenesis-Related 1 (PR1) gene expression. Collectively, these data highlight an important role for MATE proteins in the establishment of plant disease resistance.

Original languageEnglish
Pages (from-to)471-482
Number of pages12
JournalNew Phytologist
Volume192
Issue number2
DOIs
Publication statusPublished - Oct 2011

Keywords

  • activation tagging
  • biotic stress and defence signalling
  • disease resistance
  • MATE protein
  • plant defence

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