Advanced intravital subcellular imaging reveals vital three-dimensional signalling events driving cancer cell behaviour and drug responses in live tissue

Max Nobis, Neil O. Carragher, Ewan J. McGhee, Jennifer P. Morton, Owen J. Sansom, Kurt I. Anderson, Paul Timpson

Research output: Contribution to journalLiterature reviewpeer-review

Abstract

The integration of signal transduction pathways plays a fundamental role in governing disease initiation, progression and outcome. It is therefore necessary to understand disease at the signalling level to enable effective treatment and to intervene in its progression. The recent extension of invitro subcellular image-based analysis to live invivo modelling of disease is providing a more complete picture of real-time, dynamic signalling processes or drug responses in live tissue. Intravital imaging offers alternative strategies for studying disease and embraces the biological complexities that govern disease progression. In the present review, we highlight how three-dimensional or live intravital imaging has uncovered novel insights into biological mechanisms or modes of drug action. Furthermore, we offer a prospective view of how imaging applications may be integrated further with the aim of understanding disease in a more physiological and functional manner within the framework of the drug discovery process.

Original languageEnglish
Pages (from-to)5177-5197
Number of pages21
JournalFebs Journal
Volume280
Issue number21
DOIs
Publication statusPublished - Nov 2013

Keywords

  • adhesions
  • biosensors
  • drug discovery
  • fluorescence lifetime imaging microscopy
  • fluorescence recovery after photobleaching
  • fluorescence resonance energy transfer
  • intravital imaging
  • invasion
  • metastasis
  • optical windows
  • GREEN FLUORESCENT PROTEIN
  • RESONANCE ENERGY-TRANSFER
  • RHO-FAMILY GTPASES
  • LGR5 STEM-CELLS
  • IN-VIVO
  • REAL-TIME
  • PANCREATIC-CANCER
  • MAMMARY-TUMORS
  • E-CADHERIN
  • MULTIPHOTON MICROSCOPY

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