Abstract / Description of output
Enterobacter cloacae PB2 was originally isolated on the basis of its ability to utilize nitrate esters, such as pentaerythritol tebanitrate (PETN) and glycerol trinitrate, as the sole nitrogen source for growth. The enzyme responsible is an NADPH-dependent reductase designated PETN reductase. E. cloacae PB2 was found to be capable of slow aerobic growth with 2,4,6-trinitrotoluene (TNT) as the sole nitrogen source. Dinitrotoluenes mere not produced and could not be used as nitrogen sources. Purified PETN reductase,vas found to reduce TNT to its hydride-Meisenheimer complex, which was further reduced to the dihydride-Meisenheimer complex. Purified PETN reductase and recombinant Escherichia coli expressing PETN reductase were able to liberate nitrogen as nit-rite from TNT, The ability to remove nitrogen from TNT suggests that PB2 or recombinant organisms expressing PETN reductase may be useful for bioremediation of TNT-contaminated soil and water.
Original language | English |
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Pages (from-to) | 2864-2868 |
Number of pages | 5 |
Journal | Applied and Environmental Microbiology |
Volume | 64 |
Issue number | 8 |
Publication status | Published - Aug 1998 |