Alloreactive bovine T lymphocyte clones: an analysis of function, phenotype, and specificity

A.J. Teale, C L Baldwin, J A Ellis, J Newson, B M Goddeeris, Ivan Morrison

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

A bovine alloreactive cell population was subjected to complement-dependent lysis with monoclonal antibody (mAb) IL-A11. The original population and the population depleted of cells bearing the determinant recognized by mAb IL-A11 were cloned. Parent cultures and 21 clones were examined for cytolytic function and for expression of determinants recognized by mAb IL-A11 and two additional mAb, IL-A12 and IL-A17. Clones could be classified according to maximal achievable levels of cytolysis by using Theileria parva-infected bovine lymphoblastoid target cells. In this way, three groups were identified--one capable of high level cytolysis, one of intermediate levels, and one group comprising apparently noncytolytic clones. The clones in the first group reacted with mAb IL-A17; those in the second and third groups, with mAb IL-A11 and IL-A12. It was shown that cytotoxicity effected by IL-A17+ clones could be inhibited by this mAb and also by a mAb directed to MHC class I determinants on target cells. Conversely, cytotoxicity effected by IL-A11+/IL-A12+ clones could be inhibited by mAb IL-A11 and by a mAb directed to MHC class II determinants on target cells. The levels of expression of class I and class II determinants on target cells correlated with the levels of killing by clones of the IL-A17+ phenotype and clones of the IL-A11+/IL-A12+ phenotype, respectively. The results indicate that cytotoxic bovine T lymphocyte clones specific for class I MHC antigens and both cytotoxic and noncytotoxic clones specific for class II MHC antigens can be obtained. Further, their specificity for class I or class II antigens can be determined by phenotyping with mAb.
Original languageEnglish
Pages (from-to)4392-8
Number of pages7
JournalThe Journal of Immunology
Issue number12
Publication statusPublished - 15 Jun 1986

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Antibodies, Monoclonal/physiology
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface/analysis
  • Binding Sites, Antibody
  • Binding, Competitive
  • Cattle/immunology
  • Clone Cells/immunology
  • Cytotoxicity, Immunologic
  • Epitopes/analysis
  • Histocompatibility Antigens/analysis
  • Histocompatibility Antigens Class II/analysis
  • Phenotype
  • T-Lymphocytes, Cytotoxic/classification
  • T-Lymphocytes, Cytotoxic/immunology


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