Altered primordial germ cell migration in the absence of transforming growth factor beta signaling via ALK5

Susana M Chuva de Sousa Lopes; Sander van den Driesche; Rita L C Carvalho; Jonas Larsson; Bart Eggen; M Azim Surani; Christine L Mummery

doi:10.1016/j.ydbio.2005.05.019

Altered primordial germ cell migration in the absence of transforming growth factor beta signaling via ALK5

Susana M Chuva de Sousa Lopes, Sander van den Driesche, Rita L C Carvalho, Jonas Larsson, Bart Eggen, M Azim Surani, Christine L Mummery

Deanery of Clinical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Transforming growth factor beta (TGFbeta) inhibits proliferation and promotes the migration of primordial germ cells (PGCs) towards explants of gonadal ridges in vitro. However, its effects in vivo are still unclear. Here, we analyzed the behavior of PGCs in embryos lacking TGFbeta signaling via the type I receptor ALK5. TGFbeta in vivo was neither a chemoattractant for PGCs, nor did it affect their proliferation during migration towards the gonadal ridges up to embryonic day (E)10. Unexpectedly, the absence of TGFbeta signaling in fact resulted in significant facilitation of PGC migration out of the hindgut, due to the reduced deposition of collagen type I surrounding the gut of Alk5-deficient mutant embryos. Migratory PGCs adhere strongly to collagen; therefore, reduced collagen type I along the gut may result in reduced adhesion, facilitating migration into the dorsal mesenterium and gonadal ridges. Our results provide new evidence for the role of TGFbeta signaling in migration of PGCs in vivo distinct from that described previously.

Original language	English
Pages (from-to)	194-203
Number of pages	10
Journal	Developmental Biology
Volume	284
Issue number	1
DOIs	https://doi.org/10.1016/j.ydbio.2005.05.019
Publication status	Published - 1 Aug 2005

Keywords / Materials (for Non-textual outputs)

Activin Receptors, Type I
Animals
Blotting, Western
Cell Adhesion
Cell Movement
Collagen Type I
DNA Primers
Embryonic Development
Fluorescent Antibody Technique
Germ Cells
Immunohistochemistry
Mice
Protein-Serine-Threonine Kinases
Receptors, Transforming Growth Factor beta
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Transforming Growth Factor beta

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10.1016/j.ydbio.2005.05.019

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title = "Altered primordial germ cell migration in the absence of transforming growth factor beta signaling via ALK5",

abstract = "Transforming growth factor beta (TGFbeta) inhibits proliferation and promotes the migration of primordial germ cells (PGCs) towards explants of gonadal ridges in vitro. However, its effects in vivo are still unclear. Here, we analyzed the behavior of PGCs in embryos lacking TGFbeta signaling via the type I receptor ALK5. TGFbeta in vivo was neither a chemoattractant for PGCs, nor did it affect their proliferation during migration towards the gonadal ridges up to embryonic day (E)10. Unexpectedly, the absence of TGFbeta signaling in fact resulted in significant facilitation of PGC migration out of the hindgut, due to the reduced deposition of collagen type I surrounding the gut of Alk5-deficient mutant embryos. Migratory PGCs adhere strongly to collagen; therefore, reduced collagen type I along the gut may result in reduced adhesion, facilitating migration into the dorsal mesenterium and gonadal ridges. Our results provide new evidence for the role of TGFbeta signaling in migration of PGCs in vivo distinct from that described previously.",

keywords = "Activin Receptors, Type I, Animals, Blotting, Western, Cell Adhesion, Cell Movement, Collagen Type I, DNA Primers, Embryonic Development, Fluorescent Antibody Technique, Germ Cells, Immunohistochemistry, Mice, Protein-Serine-Threonine Kinases, Receptors, Transforming Growth Factor beta, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Transforming Growth Factor beta",

author = "{Chuva de Sousa Lopes}, {Susana M} and {van den Driesche}, Sander and Carvalho, {Rita L C} and Jonas Larsson and Bart Eggen and Surani, {M Azim} and Mummery, {Christine L}",

year = "2005",

month = aug,

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doi = "10.1016/j.ydbio.2005.05.019",

language = "English",

volume = "284",

pages = "194--203",

journal = "Developmental Biology",

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publisher = "Academic Press",

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T1 - Altered primordial germ cell migration in the absence of transforming growth factor beta signaling via ALK5

AU - Chuva de Sousa Lopes, Susana M

AU - van den Driesche, Sander

AU - Carvalho, Rita L C

AU - Larsson, Jonas

AU - Eggen, Bart

AU - Surani, M Azim

AU - Mummery, Christine L

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Transforming growth factor beta (TGFbeta) inhibits proliferation and promotes the migration of primordial germ cells (PGCs) towards explants of gonadal ridges in vitro. However, its effects in vivo are still unclear. Here, we analyzed the behavior of PGCs in embryos lacking TGFbeta signaling via the type I receptor ALK5. TGFbeta in vivo was neither a chemoattractant for PGCs, nor did it affect their proliferation during migration towards the gonadal ridges up to embryonic day (E)10. Unexpectedly, the absence of TGFbeta signaling in fact resulted in significant facilitation of PGC migration out of the hindgut, due to the reduced deposition of collagen type I surrounding the gut of Alk5-deficient mutant embryos. Migratory PGCs adhere strongly to collagen; therefore, reduced collagen type I along the gut may result in reduced adhesion, facilitating migration into the dorsal mesenterium and gonadal ridges. Our results provide new evidence for the role of TGFbeta signaling in migration of PGCs in vivo distinct from that described previously.

AB - Transforming growth factor beta (TGFbeta) inhibits proliferation and promotes the migration of primordial germ cells (PGCs) towards explants of gonadal ridges in vitro. However, its effects in vivo are still unclear. Here, we analyzed the behavior of PGCs in embryos lacking TGFbeta signaling via the type I receptor ALK5. TGFbeta in vivo was neither a chemoattractant for PGCs, nor did it affect their proliferation during migration towards the gonadal ridges up to embryonic day (E)10. Unexpectedly, the absence of TGFbeta signaling in fact resulted in significant facilitation of PGC migration out of the hindgut, due to the reduced deposition of collagen type I surrounding the gut of Alk5-deficient mutant embryos. Migratory PGCs adhere strongly to collagen; therefore, reduced collagen type I along the gut may result in reduced adhesion, facilitating migration into the dorsal mesenterium and gonadal ridges. Our results provide new evidence for the role of TGFbeta signaling in migration of PGCs in vivo distinct from that described previously.

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KW - Animals

KW - Blotting, Western

KW - Cell Adhesion

KW - Cell Movement

KW - Collagen Type I

KW - DNA Primers

KW - Embryonic Development

KW - Fluorescent Antibody Technique

KW - Germ Cells

KW - Immunohistochemistry

KW - Mice

KW - Protein-Serine-Threonine Kinases

KW - Receptors, Transforming Growth Factor beta

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Signal Transduction

KW - Transforming Growth Factor beta

U2 - 10.1016/j.ydbio.2005.05.019

DO - 10.1016/j.ydbio.2005.05.019

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C2 - 15993878

SN - 0012-1606

VL - 284

SP - 194

EP - 203

JO - Developmental Biology

JF - Developmental Biology

IS - 1

ER -

Altered primordial germ cell migration in the absence of transforming growth factor beta signaling via ALK5

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Keywords / Materials (for Non-textual outputs)

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