Alz-50 recognizes epitopes in primary sensory fibres and in neurons of the substantia gelatinosa of the spinal cord. An ultrastructural study in the rat

P Liberini, P Piccardo, W Ma, V Mattiuzzo, A C Cuello, A Ribeiro-Da-Silva

Research output: Contribution to journalArticlepeer-review

Abstract

The monoclonal antibody Alz-50 has been proposed as a marker for cellular pathological changes in Alzheimer's disease. However, it has been reported that this antibody also reacts with specific epitopes in normal individuals. Furthermore, intense Alz-50 immunoreactivity has been recently described in the hypothalamus and spinal cord of rat and monkey. In the present study, we analysed the distribution pattern of Alz-50 immunostaining in the spinal cord of the adult rat. Using light microscopy, immunostained fibres and varicosities were detected mainly in laminae I-II, although some immunostaining lamina I and the outer two thirds of lamina II. The varicosities appeared either scalloped or dome-shaped and contained numerous agranular synaptic vesicles and a few dense-core vesicles. Most varicosities were presynaptic to dendrites. A few immunostained cell bodies and dendrites were also observed, but glial cells were never immunostained. Some ultrathin sections were processed for postembedding immunogold detection of calcitonin gene-related peptide and GABA immunoreactivities. Most of the varicosities which were immunoreactive for Alz-50 also showed calcitonin gene-related peptide immunoreactivity. In contrast, GABA immunoreactivity was never co-localized with Alz-50 immunoreactivity. These results indicate that, in the superficial dorsal horn, the epitope recognized by the Alz-50 antibody is located mainly, but not exclusively, in primary sensory fibres.

Original languageEnglish
Pages (from-to)559-67
Number of pages9
JournalJournal of neurocytology
Volume24
Issue number8
Publication statusPublished - Aug 1995

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Calcitonin Gene-Related Peptide
  • Cell Membrane
  • Dendrites
  • Epitopes
  • Immunohistochemistry
  • Intermediate Filaments
  • Male
  • Microscopy, Electron
  • Microtubules
  • Mitochondria
  • Nerve Fibers
  • Neurons
  • Rats
  • Rats, Wistar
  • Substantia Gelatinosa
  • gamma-Aminobutyric Acid

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