Amino-terminal sequencing of sheep CD1 antigens and identification of a sheep CD1D gene

S M Rhind, J Hopkins, B M Dutia

Research output: Contribution to journalArticlepeer-review

Abstract

The anti-CD1 monoclonal antibodies IAH-CC14 and SBU-T6 were used to immunopurify CD1 antigens from sheep thymocytes. The amino-terminal sequence of IAH-CC14 yielded 13 amino acids, and 29 amino acids were obtained from the SBU-T6 antigen. The sequence of the IAH-CC14 antigen was 100% identical to the predicted sequence of the sheep CD1B clone, SCD1B-42. The 29 amino acid sequence of the SBU-T6 antigen did not match identically with the derived amino acid sequence of any of the previously reported sheep CD1 genes but had closest similarity to the derived sequence of human CD1E. Degenerate polymerase chain reaction primers based on this sequence identified a group 2 sheep CD1 gene. The predicted amino acid sequence of this gene shows that it is not identical to the SBU-T6 peptide, indicating that a different, CD1D-like gene was cloned.
Original languageEnglish
Pages (from-to)225-30
Number of pages6
JournalImmunogenetics
Volume49
Issue number3
Publication statusPublished - Mar 1999

Keywords

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Antigens, CD1
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Exons
  • Humans
  • Introns
  • Molecular Sequence Data
  • Molecular Weight
  • Polymerase Chain Reaction
  • Precipitin Tests
  • Sequence Alignment
  • Sequence Analysis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sheep
  • T-Lymphocytes

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