TY - JOUR
T1 - Amperometric IFN-γ immunosensors with commercially fabricated PCB sensing electrodes
AU - Moschou, Despina
AU - Greathead, Louise
AU - Pantelidis, Panagiotis
AU - Kelleher, Peter
AU - Morgan, Hywel
AU - Prodromakis, Themistoklis
PY - 2016/12/15
Y1 - 2016/12/15
N2 - Lab-on-a-Chip (LoC) technology has the potential to revolutionize medical Point-of-Care diagnostics. Currently, considerable research efforts are focused on innovative production technologies that will make commercial upscaling of lab-on-chip products financially viable. Printed circuit board (PCB) manufacturing techniques have several advantages in this field. In this paper we focus on transferring a complete IFN-γ enzyme-linked immune-sorbent assay (ELISA) onto a commercial PCB electrochemical biosensing platform, We adapted a commercially available ELISA to detect the enzyme product TMB/H2O2 using amperometry, successfully reproducing the colorimetry-obtained ELISA standard curve. The results demonstrate the potential for the integration of these components into an automated, disposable, electronic ELISA Lab-on-PCB diagnostic platform.
AB - Lab-on-a-Chip (LoC) technology has the potential to revolutionize medical Point-of-Care diagnostics. Currently, considerable research efforts are focused on innovative production technologies that will make commercial upscaling of lab-on-chip products financially viable. Printed circuit board (PCB) manufacturing techniques have several advantages in this field. In this paper we focus on transferring a complete IFN-γ enzyme-linked immune-sorbent assay (ELISA) onto a commercial PCB electrochemical biosensing platform, We adapted a commercially available ELISA to detect the enzyme product TMB/H2O2 using amperometry, successfully reproducing the colorimetry-obtained ELISA standard curve. The results demonstrate the potential for the integration of these components into an automated, disposable, electronic ELISA Lab-on-PCB diagnostic platform.
KW - Amperometric biosensor
KW - Immunosensors
KW - Lab-on-PCB
KW - PCB sensors
UR - http://www.scopus.com/inward/record.url?scp=84979955423&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2016.07.075
DO - 10.1016/j.bios.2016.07.075
M3 - Article
C2 - 27479047
AN - SCOPUS:84979955423
VL - 86
SP - 805
EP - 810
JO - Biosensors & Bioelectronics
JF - Biosensors & Bioelectronics
SN - 0956-5663
ER -