TY - JOUR
T1 - Análise morfométrica comparada entre Anuros endêmicos do Brasil e a espécie invasora Lithobates catesbeianus
AU - Silva, Stephanie Moira Rodrigues e.
AU - Ewbank, Ana Carolina
AU - Strefezzi, Ricardo de Francisco
AU - Alvarado, Gilbert
AU - Sacristan, Carlos
AU - de Paula, Cátia Dejuste
AU - Catão-Dias, José Luiz
N1 - Funding Information:
We thank J. Oyakawa, at Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, for his technical support and collaboration. This work was supported by the Coordination for the Improvement of Higher Level Personnel (CAPES) and by the National Counsel of Technological and Scientific Development (CNPQ) (grant number 138966/2010–9).
Publisher Copyright:
© 2017, Universidade de Sao Paulo. Faculdade de Medicina Veterinaria e Zootecnia. All rights reserved.
PY - 2017/8/18
Y1 - 2017/8/18
N2 - Amphibians are potentially reliable and efficient bioindicators. Existing anuran white blood cell morphology studies are limited, with only a few morphometric studies available. We employed morphometric techniques to characterize leukocytes of selected Neotropical anurans from Brazil and compare our findings with the exotic American Bullfrog (Lithobates catesbeianus), genus Ranidae. We compared blood smears of 28 specimens from six different genera (Hyla, Phyllomedusa, Hypsiboas, Scinax, Physalaemus, and Proceratophrys) with samples from L. catesbeianus. Leukocyte average diameter was calculated by an image analysis software. One-way analyses of variance and Bonferroni tests were used on statistical analyses. Lymphocytes, neutrophils, eosinophils, and basophils were significantly smaller than the reference ranges reported for other amphibian genera, including Lithobathes, whereas monocyte diameters did not differ significantly between genera. This is the first study to evaluate leukocyte morphometrics of Brazilian anuran species. Our findings suggest that geographical separation could possibly influence leukocyte morphometry.
AB - Amphibians are potentially reliable and efficient bioindicators. Existing anuran white blood cell morphology studies are limited, with only a few morphometric studies available. We employed morphometric techniques to characterize leukocytes of selected Neotropical anurans from Brazil and compare our findings with the exotic American Bullfrog (Lithobates catesbeianus), genus Ranidae. We compared blood smears of 28 specimens from six different genera (Hyla, Phyllomedusa, Hypsiboas, Scinax, Physalaemus, and Proceratophrys) with samples from L. catesbeianus. Leukocyte average diameter was calculated by an image analysis software. One-way analyses of variance and Bonferroni tests were used on statistical analyses. Lymphocytes, neutrophils, eosinophils, and basophils were significantly smaller than the reference ranges reported for other amphibian genera, including Lithobathes, whereas monocyte diameters did not differ significantly between genera. This is the first study to evaluate leukocyte morphometrics of Brazilian anuran species. Our findings suggest that geographical separation could possibly influence leukocyte morphometry.
KW - Anura
KW - Morphometry
KW - South America
KW - White blood cells
UR - https://www.scopus.com/pages/publications/85028704375
U2 - 10.11606/issn.1678-4456.bjvras.2017.121887
DO - 10.11606/issn.1678-4456.bjvras.2017.121887
M3 - Article
AN - SCOPUS:85028704375
SN - 1413-9596
VL - 54
SP - 159
EP - 167
JO - Brazilian Journal of Veterinary research and Animal Science
JF - Brazilian Journal of Veterinary research and Animal Science
IS - 2
ER -