An efficient purification system for native minichromosome from saccharomyces cerevisiae

Ashwin Unnikrishnan, Bungo Akiyoshi, Sue Biggins, Toshio Tsukiyama*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract / Description of output

We have recently established a system for purifying minichromosomes in a native state from Saccharomyces cerevisiae. This system is extremely efficient, and a single-step purification yields samples with sufficient purity and quantity for mass spectrometry (MS) analysis of histones and non-histone proteins tightly associated with the minichromosome. The templates can also be used in various biochemical assays in vitro, such as transcription and recombination, and could be suitable for EM or other biophysical studies.

Original languageEnglish
Title of host publicationChromatin Remodeling
Subtitle of host publicationMethods and Protocols
EditorsRandall H. Morse
PublisherHumana Press
Chapter8
Pages115-123
Number of pages9
Edition1
ISBN (Electronic)9781617794773
ISBN (Print)9781617794766
DOIs
Publication statusPublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume833
ISSN (Print)1064-3745

Keywords / Materials (for Non-textual outputs)

  • Chromatin
  • DNA replication
  • Episome
  • FLAG tag
  • Histone modifications
  • Mass spectrometry
  • Minichromosome
  • Purification
  • Replication origin
  • TRP1-ARS1

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