An efficient purification system for native minichromosome from saccharomyces cerevisiae

Ashwin Unnikrishnan; Bungo Akiyoshi; Sue Biggins; Toshio Tsukiyama

doi:10.1007/978-1-61779-477-3_8

An efficient purification system for native minichromosome from saccharomyces cerevisiae

Ashwin Unnikrishnan, Bungo Akiyoshi, Sue Biggins, Toshio Tsukiyama^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

We have recently established a system for purifying minichromosomes in a native state from Saccharomyces cerevisiae. This system is extremely efficient, and a single-step purification yields samples with sufficient purity and quantity for mass spectrometry (MS) analysis of histones and non-histone proteins tightly associated with the minichromosome. The templates can also be used in various biochemical assays in vitro, such as transcription and recombination, and could be suitable for EM or other biophysical studies.

Original language	English
Title of host publication	Chromatin Remodeling
Subtitle of host publication	Methods and Protocols
Editors	Randall H. Morse
Publisher	Humana Press
Chapter	8
Pages	115-123
Number of pages	9
Edition	1
ISBN (Electronic)	9781617794773
ISBN (Print)	9781617794766
DOIs	https://doi.org/10.1007/978-1-61779-477-3_8
Publication status	Published - 2012
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	833
ISSN (Print)	1064-3745

Keywords / Materials (for Non-textual outputs)

Chromatin
DNA replication
Episome
FLAG tag
Histone modifications
Mass spectrometry
Minichromosome
Purification
Replication origin
TRP1-ARS1

Access to Document

10.1007/978-1-61779-477-3_8

Cite this

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abstract = "We have recently established a system for purifying minichromosomes in a native state from Saccharomyces cerevisiae. This system is extremely efficient, and a single-step purification yields samples with sufficient purity and quantity for mass spectrometry (MS) analysis of histones and non-histone proteins tightly associated with the minichromosome. The templates can also be used in various biochemical assays in vitro, such as transcription and recombination, and could be suitable for EM or other biophysical studies.",

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An efficient purification system for native minichromosome from saccharomyces cerevisiae. / Unnikrishnan, Ashwin; Akiyoshi, Bungo; Biggins, Sue et al.
Chromatin Remodeling: Methods and Protocols. ed. / Randall H. Morse. 1. ed. Humana Press, 2012. p. 115-123 (Methods in Molecular Biology; Vol. 833).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

TY - CHAP

T1 - An efficient purification system for native minichromosome from saccharomyces cerevisiae

AU - Unnikrishnan, Ashwin

AU - Akiyoshi, Bungo

AU - Biggins, Sue

AU - Tsukiyama, Toshio

PY - 2012

Y1 - 2012

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AB - We have recently established a system for purifying minichromosomes in a native state from Saccharomyces cerevisiae. This system is extremely efficient, and a single-step purification yields samples with sufficient purity and quantity for mass spectrometry (MS) analysis of histones and non-histone proteins tightly associated with the minichromosome. The templates can also be used in various biochemical assays in vitro, such as transcription and recombination, and could be suitable for EM or other biophysical studies.

KW - Chromatin

KW - DNA replication

KW - Episome

KW - FLAG tag

KW - Histone modifications

KW - Mass spectrometry

KW - Minichromosome

KW - Purification

KW - Replication origin

KW - TRP1-ARS1

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SN - 9781617794766

T3 - Methods in Molecular Biology

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BT - Chromatin Remodeling

A2 - Morse, Randall H.

PB - Humana Press

ER -

An efficient purification system for native minichromosome from saccharomyces cerevisiae

Abstract

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Keywords / Materials (for Non-textual outputs)

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