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Abstract
Most human genes are associated with promoters embedded in non-methylated, G + C-rich CpG islands (CGIs). Not all CGIs are found at annotated promoters, however, raising the possibility that many serve as promoters for transcripts that do not code for proteins. To test this hypothesis, we searched for novel transcripts in embryonic stem cells (ESCs) that originate within orphan CGIs. Among several candidates, we detected a transcript that included three members of the let-7 micro-RNA family: Let-7a-1, let-7f-1, and let-7d. Deletion of the CGI prevented expression of the precursor RNA and depleted the included miRNAs. Mice homozygous for this mutation were sub-viable and showed growth and other defects. The results suggest that despite the identity of their seed sequences, members of the let-7 miRNA family exert distinct functions that cannot be complemented by other members.
Original language | English |
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Number of pages | 14 |
Journal | Epigenomes |
Volume | 3 |
Issue number | 1 |
DOIs | |
Publication status | Published - 13 Mar 2019 |
Keywords / Materials (for Non-textual outputs)
- micro-RNA
- Let-7
- mouse genetics
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Dive into the research topics of 'An Orphan CpG Island Drives Expression of a let-7 miRNA Precursor with an Important Role in Mouse Development'. Together they form a unique fingerprint.Projects
- 1 Finished
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Towards understanding and treatment of MeCP2-related disorders
Bird, A. (Principal Investigator)
1/01/16 → 30/06/22
Project: Research