An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei

A Schnaufer; A K Panigrahi; B Panicucci; R P Igo; E Wirtz; R Salavati; K Stuart

doi:10.1126/science.1058955

An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei

A Schnaufer, A K Panigrahi, B Panicucci, R P Igo, E Wirtz, R Salavati, K Stuart

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

RNA editing in trypanosomes occurs by a series of enzymatic steps that are catalyzed by a macromolecular complex. The TbMP52 protein is shown to be a component of this complex, to have RNA ligase activity, and to be one of two adenylatable proteins in the complex. Regulated repression of TbMP52 blocks editing, which shows that it is a functional component of the editing complex. This repression is lethal in bloodforms of the parasite, indicating that editing is essential in the mammalian stage of the life cycle. The editing complex, which is present in all kinetoplastid parasites, may thus be a chemotherapeutic target.

Original language	English
Pages (from-to)	2159-62
Number of pages	4
Journal	Science
Volume	291
Issue number	5511
DOIs	https://doi.org/10.1126/science.1058955
Publication status	Published - 2001

Access to Document

10.1126/science.1058955

Cite this

@article{e95e6b807c39409bbda85db6e6f3e52b,

title = "An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei",

abstract = "RNA editing in trypanosomes occurs by a series of enzymatic steps that are catalyzed by a macromolecular complex. The TbMP52 protein is shown to be a component of this complex, to have RNA ligase activity, and to be one of two adenylatable proteins in the complex. Regulated repression of TbMP52 blocks editing, which shows that it is a functional component of the editing complex. This repression is lethal in bloodforms of the parasite, indicating that editing is essential in the mammalian stage of the life cycle. The editing complex, which is present in all kinetoplastid parasites, may thus be a chemotherapeutic target.",

author = "A Schnaufer and Panigrahi, {A K} and B Panicucci and Igo, {R P} and E Wirtz and R Salavati and K Stuart",

year = "2001",

doi = "10.1126/science.1058955",

language = "English",

volume = "291",

pages = "2159--62",

journal = "Science",

issn = "0036-8075",

publisher = "AMER ASSOC ADVANCEMENT SCIENCE",

number = "5511",

}

TY - JOUR

T1 - An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei

AU - Schnaufer, A

AU - Panigrahi, A K

AU - Panicucci, B

AU - Igo, R P

AU - Wirtz, E

AU - Salavati, R

AU - Stuart, K

PY - 2001

Y1 - 2001

N2 - RNA editing in trypanosomes occurs by a series of enzymatic steps that are catalyzed by a macromolecular complex. The TbMP52 protein is shown to be a component of this complex, to have RNA ligase activity, and to be one of two adenylatable proteins in the complex. Regulated repression of TbMP52 blocks editing, which shows that it is a functional component of the editing complex. This repression is lethal in bloodforms of the parasite, indicating that editing is essential in the mammalian stage of the life cycle. The editing complex, which is present in all kinetoplastid parasites, may thus be a chemotherapeutic target.

AB - RNA editing in trypanosomes occurs by a series of enzymatic steps that are catalyzed by a macromolecular complex. The TbMP52 protein is shown to be a component of this complex, to have RNA ligase activity, and to be one of two adenylatable proteins in the complex. Regulated repression of TbMP52 blocks editing, which shows that it is a functional component of the editing complex. This repression is lethal in bloodforms of the parasite, indicating that editing is essential in the mammalian stage of the life cycle. The editing complex, which is present in all kinetoplastid parasites, may thus be a chemotherapeutic target.

U2 - 10.1126/science.1058955

DO - 10.1126/science.1058955

M3 - Article

C2 - 11251122

SN - 0036-8075

VL - 291

SP - 2159

EP - 2162

JO - Science

JF - Science

IS - 5511

ER -

An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei

Abstract / Description of output

Access to Document

Fingerprint

Cite this