An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei

Appolinaire Djikeng; Huafang Shi; Christian Tschudi; Shuiyuan Shen; Elisabetta Ullu

doi:10.1261/rna.5270203

An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei

Appolinaire Djikeng, Huafang Shi, Christian Tschudi, Shuiyuan Shen, Elisabetta Ullu^*

^*Corresponding author for this work

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Genetic interference by double-stranded RNA (dsRNA) or RNA interference (RNAi) triggers the sequence-specific degradation of cellular transcripts. The mediators of mRNA degradation are small interfering RNAs (siRNAs). Here, we report that in Trypanosoma brucei, 10%-20% of siRNAs cosediment with polyribosomes. Preventing the assembly of ribosomes on mRNAs results in a concomitant decrease of siRNAs sedimenting as high-molecular-weight complexes. We further provide evidence that siRNAs are associated with translating ribosomes and that this association is mediated by a 70-kD ribonucleoprotein complex.

Original language	English
Pages (from-to)	802-808
Number of pages	7
Journal	RNA
Volume	9
Issue number	7
DOIs	https://doi.org/10.1261/rna.5270203
Publication status	Published - 1 Jul 2003

Keywords / Materials (for Non-textual outputs)

Ribonucleoprotein
RNAi
siRNAs, polyribosomes
Trypanosoma brucei

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10.1261/rna.5270203

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title = "An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei",

abstract = "Genetic interference by double-stranded RNA (dsRNA) or RNA interference (RNAi) triggers the sequence-specific degradation of cellular transcripts. The mediators of mRNA degradation are small interfering RNAs (siRNAs). Here, we report that in Trypanosoma brucei, 10%-20% of siRNAs cosediment with polyribosomes. Preventing the assembly of ribosomes on mRNAs results in a concomitant decrease of siRNAs sedimenting as high-molecular-weight complexes. We further provide evidence that siRNAs are associated with translating ribosomes and that this association is mediated by a 70-kD ribonucleoprotein complex.",

keywords = "Ribonucleoprotein, RNAi, siRNAs, polyribosomes, Trypanosoma brucei",

author = "Appolinaire Djikeng and Huafang Shi and Christian Tschudi and Shuiyuan Shen and Elisabetta Ullu",

year = "2003",

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doi = "10.1261/rna.5270203",

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T1 - An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei

AU - Djikeng, Appolinaire

AU - Shi, Huafang

AU - Tschudi, Christian

AU - Shen, Shuiyuan

AU - Ullu, Elisabetta

PY - 2003/7/1

Y1 - 2003/7/1

N2 - Genetic interference by double-stranded RNA (dsRNA) or RNA interference (RNAi) triggers the sequence-specific degradation of cellular transcripts. The mediators of mRNA degradation are small interfering RNAs (siRNAs). Here, we report that in Trypanosoma brucei, 10%-20% of siRNAs cosediment with polyribosomes. Preventing the assembly of ribosomes on mRNAs results in a concomitant decrease of siRNAs sedimenting as high-molecular-weight complexes. We further provide evidence that siRNAs are associated with translating ribosomes and that this association is mediated by a 70-kD ribonucleoprotein complex.

AB - Genetic interference by double-stranded RNA (dsRNA) or RNA interference (RNAi) triggers the sequence-specific degradation of cellular transcripts. The mediators of mRNA degradation are small interfering RNAs (siRNAs). Here, we report that in Trypanosoma brucei, 10%-20% of siRNAs cosediment with polyribosomes. Preventing the assembly of ribosomes on mRNAs results in a concomitant decrease of siRNAs sedimenting as high-molecular-weight complexes. We further provide evidence that siRNAs are associated with translating ribosomes and that this association is mediated by a 70-kD ribonucleoprotein complex.

KW - Ribonucleoprotein

KW - RNAi

KW - siRNAs, polyribosomes

KW - Trypanosoma brucei

UR - http://www.scopus.com/inward/record.url?scp=0038721055&partnerID=8YFLogxK

U2 - 10.1261/rna.5270203

DO - 10.1261/rna.5270203

M3 - Article

C2 - 12810914

AN - SCOPUS:0038721055

SN - 1355-8382

VL - 9

SP - 802

EP - 808

JO - RNA

JF - RNA

IS - 7

ER -

An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei

Abstract

Keywords / Materials (for Non-textual outputs)

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