An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei

Appolinaire Djikeng, Huafang Shi, Christian Tschudi, Shuiyuan Shen, Elisabetta Ullu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Genetic interference by double-stranded RNA (dsRNA) or RNA interference (RNAi) triggers the sequence-specific degradation of cellular transcripts. The mediators of mRNA degradation are small interfering RNAs (siRNAs). Here, we report that in Trypanosoma brucei, 10%-20% of siRNAs cosediment with polyribosomes. Preventing the assembly of ribosomes on mRNAs results in a concomitant decrease of siRNAs sedimenting as high-molecular-weight complexes. We further provide evidence that siRNAs are associated with translating ribosomes and that this association is mediated by a 70-kD ribonucleoprotein complex.

Original languageEnglish
Pages (from-to)802-808
Number of pages7
JournalRNA
Volume9
Issue number7
DOIs
Publication statusPublished - 1 Jul 2003

Keywords

  • Ribonucleoprotein
  • RNAi
  • siRNAs, polyribosomes
  • Trypanosoma brucei

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