Analysis of cardiac function by MRI and stereology

N Roberts, LM CruzOrive, M Bourne, RJ Herfkens, RA Karwoski, GH Whitehouse

Research output: Contribution to journalArticlepeer-review

Abstract

Design-based stereology and phase contrast magnetic resonance imaging (MRI) were combined to monitor changes in the volume of the four chambers of the human heart during the cardiac cycle, The data set consisted of 18 adjacent slices (or 'scanning levels') of 0.5 cm thickness, perpendicular to the long axis of the body, and encompassing the whole heart of a healthy volunteer, At each scanning level, a cardiac gated MR image was obtained at each of 16 equally spaced time frames within the cardiac cycle. Given stationarity with respect to time, absence of image artefacts and appropriate definition of chamber boundaries, for each time frame unbiased estimates of total blood volume in the relevant heart chambers were efficiently obtained using the Cavalieri method and point counting, Combined with a proper MRI acquisition, modern stereological methods constitute an efficient and reliable tool to quantify cardiac function noninvasively.

Original languageEnglish
Pages (from-to)31-42
Number of pages12
JournalJournal of Microscopy
Volume187
Publication statusPublished - Jul 1997

Keywords

  • cardiology
  • Cavalieri method
  • heart
  • magnetic resonance imaging (MRI)
  • point counting
  • stereology
  • systematic sampling
  • volume
  • TOTAL VERTICAL PROJECTIONS
  • MAGNETIC-RESONANCE
  • SURFACE-AREA
  • EFFICIENT ESTIMATION
  • CAVALIERI PRINCIPLE
  • UNBIASED ESTIMATION
  • EJECTION FRACTION
  • VOLUME
  • SECTIONS
  • BLADDER

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