@inbook{840fed8c93f0426e8cdcb8b9f3790b99,
title = "Analysis of DNA-Protein Interactions Using PAGE: Band-Shift Assays",
abstract = "The band-shift assay using polyacrylamide gel electrophoresis is a powerful technique used to investigate DNA-protein interactions. The basis of the method is the separation of free DNA from DNA-protein complexes by virtue of differences in charge, size, and shape. The band-shift assay can be used to determine thermodynamic and kinetic binding constants and also to analyze the composition and stoichiometries of DNA-protein complexes.",
keywords = "Band shift, DNA-protein interaction, DNA binding constant, EMSA, PAGE, Gel retardation, NUCLEIC ACID INTERACTIONS, GEL-ELECTROPHORESIS, BINDING, METHYLTRANSFERASE, METHIONINE, KINETICS, ECOKI",
author = "Lynn Powell",
year = "2013",
doi = "10.1007/978-1-62703-565-1_16",
language = "English",
isbn = "978-1-62703-564-4",
series = "Methods in Molecular Biology",
publisher = "HUMANA PRESS INC",
pages = "237--251",
editor = "S Makovets",
booktitle = "DNA ELECTROPHORESIS: METHODS AND PROTOCOLS",
}