Analysis of DNA-Protein Interactions Using PAGE: Band-Shift Assays

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The band-shift assay using polyacrylamide gel electrophoresis is a powerful technique used to investigate DNA-protein interactions. The basis of the method is the separation of free DNA from DNA-protein complexes by virtue of differences in charge, size, and shape. The band-shift assay can be used to determine thermodynamic and kinetic binding constants and also to analyze the composition and stoichiometries of DNA-protein complexes.

Original languageEnglish
Title of host publicationDNA ELECTROPHORESIS: METHODS AND PROTOCOLS
EditorsS Makovets
Place of PublicationTOTOWA
PublisherHUMANA PRESS INC
Pages237-251
Number of pages15
ISBN (Print)978-1-62703-564-4
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
PublisherHUMANA PRESS INC
Volume1054
ISSN (Print)1064-3745

Keywords

  • Band shift
  • DNA-protein interaction
  • DNA binding constant
  • EMSA
  • PAGE
  • Gel retardation
  • NUCLEIC ACID INTERACTIONS
  • GEL-ELECTROPHORESIS
  • BINDING
  • METHYLTRANSFERASE
  • METHIONINE
  • KINETICS
  • ECOKI

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