Analysis of LINE-1 expression in human pluripotent cells

Martin Muñoz-Lopez, Marta Garcia-Cañadas, Angela Macia, Santiago Morell, Jose L Garcia-Perez

Research output: Contribution to journalArticlepeer-review

Abstract

Half of the human genome is composed of repeated DNA, and some types are mobile within our genome (transposons and retrotransposons). Despite their abundance, only a small fraction of them are currently active in our genome (Long Interspersed Element-1 (LINE-1), Alu, and SVA elements). LINE-1 or L1 elements are a family of active non-LTR retrotransposons, the ongoing mobilization of which still impacts our genome. As selfish DNA elements, L1 activity is more prominent in early human development, where new insertions would be transmitted to the progeny. Here, we describe the conventional methods aimed to determine the expression level of LINE-1 elements in pluripotent human cells.

Original languageEnglish
Pages (from-to)113-25
Number of pages13
JournalMethods in Molecular Biology
Volume873
DOIs
Publication statusPublished - 2012

Keywords

  • Blotting, Western
  • DNA Methylation
  • Embryonic Stem Cells
  • Humans
  • Induced Pluripotent Stem Cells
  • Long Interspersed Nucleotide Elements
  • Real-Time Polymerase Chain Reaction
  • Retroelements

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