TY - JOUR
T1 - Analysis of multidimensional G-quadruplex melting curves
AU - Gray, Robert D
AU - Chaires, Jonathan B
N1 - © 2011 by John Wiley & Sons, Inc.
PY - 2011
Y1 - 2011
N2 - Multidimensional "3D" melting curves for G-quadruplexes are obtained by recording whole spectra (absorbance, CD, fluorescence) as a function of temperature, rather than the common approach of recording the spectral response to temperature at a single wavelength. 3D melting curves are richer in information, and can be used to enumerate the number of significant species and intermediate states required to properly analyze the thermal denaturation reaction to obtain thermodynamic information. This unit describes the application of the method of singular value decomposition to the analysis of 3D melting data obtained for G-quadruplex structures, and how the results of such an analysis can be used to provide a more complete characterization of the mechanism of quadruplex unfolding.
AB - Multidimensional "3D" melting curves for G-quadruplexes are obtained by recording whole spectra (absorbance, CD, fluorescence) as a function of temperature, rather than the common approach of recording the spectral response to temperature at a single wavelength. 3D melting curves are richer in information, and can be used to enumerate the number of significant species and intermediate states required to properly analyze the thermal denaturation reaction to obtain thermodynamic information. This unit describes the application of the method of singular value decomposition to the analysis of 3D melting data obtained for G-quadruplex structures, and how the results of such an analysis can be used to provide a more complete characterization of the mechanism of quadruplex unfolding.
U2 - 10.1002/0471142700.nc1704s45
DO - 10.1002/0471142700.nc1704s45
M3 - Article
C2 - 21638271
SN - 1934-9289
VL - Chapter 17
SP - Unit17.4
JO - Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]
JF - Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]
ER -