Analysis of signalling pathways by western blotting and immunoprecipitation

Aymen I Idris

Research output: Contribution to journalArticlepeer-review

Abstract

This chapter describes the analysis of signalling pathways in bone cells by the use of western blotting and immunoprecipitation, including a step by step guide to cell culture techniques, protein isolation, purification, measurement, electrophoretic transfer, and detection.
Original languageEnglish
Pages (from-to)223-32
Number of pages10
JournalMethods in Molecular Biology
Volume816
DOIs
Publication statusPublished - 2012

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