Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

A plethora of biological processes like gene transcription, DNA replication, DNA recombination, and chromosome segregation are mediated through protein-DNA interactions. A powerful method for investigating proteins within a native chromatin environment in the cell is chromatin immunoprecipitation (ChIP). Combined with the recent technological advancement in next generation sequencing, the ChIP assay can map the exact binding sites of a protein of interest across the entire genome. Here we describe a-step-by step protocol for ChIP followed by library preparation for ChIP-seq from yeast cells.

Original languageEnglish
Title of host publicationSMC Complexes
Subtitle of host publicationMethods and Protocols
EditorsAnjana Badrinarayanan
PublisherSpringer
Pages119-138
Number of pages20
Volume2004
ISBN (Electronic)978-1-4939-9520-2
ISBN (Print)978-1-4939-9519-6
DOIs
Publication statusPublished - 31 May 2019

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Keywords

  • Chromatin immunoprecipitation
  • Saccharomyces cerevisiae
  • Schizosaccharomyces pombe
  • Cohesin
  • Condensin
  • Mitosis
  • Meiosis
  • Scc1
  • Rec8
  • Brn1

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