Analysis of the fine specificities of sheep major histocompatibility complex class II-specific monoclonal antibodies using mouse L-cell transfectants

K T Ballingall, B M Dutia, J Hopkins, H Wright

Research output: Contribution to journalArticlepeer-review

Abstract

The fine specificities of two panels of monoclonal antibodies (mAbs) for sheep major histocompatibility complex (MHC) class II molecules were determined using five mouse L-cell transfectants, each expressing a defined sheep DQ or DR MHC class II A/B gene pair. Using the transfectants in an indirect fluorescence antibody assay, previous immunochemical characterization of the mAbs was confirmed for 16 of 23 mAbs tested. The MHC class II subtype specificity (DQ or DR) of each mAb was assigned without interference from the products of other expressed class II loci. This allowed the identification of both cross-locus specificities as well as defining fine specificities of mAbs previously only partially characterized by immunochemical techniques.
Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalAnimal Genetics
Volume26
Issue number2
Publication statusPublished - Apr 1995

Keywords

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Base Sequence
  • DNA
  • Histocompatibility Antigens Class II
  • L Cells (Cell Line)
  • Major Histocompatibility Complex
  • Mice
  • Molecular Sequence Data
  • Recombinant Proteins
  • Sheep
  • Transfection

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