Analysis of the function of the photoreceptors phytochrome B and phytochrome D in Nicotiana plumbaginifolia and Arabidopsis thaliana

Aurora Piñas Fernández, Patricia Gil, Ildiko Valkai, Ferenc Nagy, Eberhard Schäfer

Research output: Contribution to journalArticlepeer-review

Abstract

To investigate the mechanism of phytochrome action in vivo, NtPHYB, AtPHYB and phyD:green fluorescent protein (GFP) were overexpressed in Nicotiana plumbaginifolia and Arabidopsis thaliana. The expression of 35S:NtPHYB:GFP and 35S:AtPHYB:GFP complemented the tobacco hgl2 and Arabidopsis phyB-9 mutations, whereas the 35S:AtPHYD:GFP only rescued the hgl2 mutant. All three fusion proteins are transported into the nucleus in all genetic backgrounds. These data indicate that AtPHYD:GFP is biologically active and functions as the main red light receptor in transgenic tobacco, and establish an experimental system for the functional analysis of this elusive photoreceptor in vivo.
Original languageEnglish
Pages (from-to)790-6
Number of pages7
JournalPlant and cell physiology
Volume46
Issue number5
DOIs
Publication statusPublished - 2005

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