Analysis of the reactivity of anti-bovine CD8 monoclonal antibodies with cloned T cell lines and mouse L-cells transfected with bovine CD8

Niall MacHugh, Albert Bensaid, Chris J Howard, W.C. Davis, Ivan Morrison

Research output: Contribution to journalArticlepeer-review

Abstract

Mouse L-cells transfected with bovine CD8 and two Theileria parva-infected cloned T cell lines expressing bovine CD8 were used to screen the panel of ten monoclonal antibodies (mAbs) submitted to the workshop. Eight of the ten mAbs reacted with the transfectant and both the cloned T cell lines. However, two mAbs CC58 and BAT82A did not recognise the transfectant and only reacted with one of the T cell lines. Further biochemical studies indicated that the eight mAbs react with both homo- and heterodimeric forms of bovine CD8 whilst the two mAbs CC58 and BAT82A react with only heterodimeric forms. These data suggest that bovine DC8 is encoded by two genes as is the case in mouse and man.
Original languageEnglish
Pages (from-to)169-72
Number of pages4
JournalVeterinary Immunology and Immunopathology
Volume27
Issue number1-3
Publication statusPublished - Jan 1991

Keywords

  • Animals
  • Antibodies, Monoclonal/immunology
  • Antigens, CD8
  • Antigens, Differentiation, T-Lymphocyte/genetics
  • Antigens, Differentiation, T-Lymphocyte/immunology
  • Apicomplexa/growth & development
  • Cattle/immunology
  • Clone Cells
  • Electrophoresis, Polyacrylamide Gel
  • Flow Cytometry
  • L Cells (Cell Line)/immunology
  • L Cells (Cell Line)/parasitology
  • Mice
  • Precipitin Tests
  • T-Lymphocytes/immunology
  • Theileriasis/immunology
  • Theileriasis/parasitology
  • Transfection

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