Analysis of the Role of TpUB05 Antigen from Theileria parva in Immune Responses to Malaria in Humans Compared to Its Homologue in Plasmodium falciparum the UB05 Antigen

Jerome Nyhalah Dinga; Stephanie Numenyi Perimbie; Stanley Dobgima   Gamua; Francis N. G.  Chuma; Dieudonne Lemuh Njimoh; Appolinaire Djikeng; Roger Pelle; Vincent Pryde Kehdingha Titanji

doi:10.3390/pathogens9040271

Analysis of the Role of TpUB05 Antigen from Theileria parva in Immune Responses to Malaria in Humans Compared to Its Homologue in Plasmodium falciparum the UB05 Antigen

Jerome Nyhalah Dinga, Stephanie Numenyi Perimbie , Stanley Dobgima Gamua, Francis N. G. Chuma, Dieudonne Lemuh Njimoh, Appolinaire Djikeng, Roger Pelle, Vincent Pryde Kehdingha Titanji

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Abstract

Despite the amount of resources deployed and the technological advancements in molecular biology, vaccinology, immunology, genetics, and biotechnology, there are still no effective vaccines against malaria. Immunity to malaria is usually seen to be species- and/or strain-specific. However, there is a growing body of evidence suggesting the possibility of the existence of cross-strain, cross-species, and cross-genus immune responses in apicomplexans. The principle of gene conservation indicates that homologues play a similar role in closely related organisms. The homologue of UB05 in Theileria parva is TpUB05 (XP_763711.1), which has been tested and shown to be associated with protective immunity in East Coast fever. In a bid to identify potent markers of protective immunity to aid malaria vaccine development, TpUB05 was tested in malaria caused by Plasmodium falciparum. It was observed that TpUB05 was better at detecting antigen-specific antibodies in plasma compared to UB05 when tested by ELISA. The total IgG raised against TpUB05 was able to block parasitic growth in vitro more effectively than that raised against UB05. However, there was no significant difference between the two study antigens in recalling peripheral blood mononuclear cell (PBMC) memory through IFN-γ production. This study suggests, for the first time, that TpUB05 from T. parva cross-reacts with UB05 from P. falciparum and is a marker of protective immunity in malaria. Hence, TpUB05 should be considered for possible development as a potential subunit vaccine candidate against malaria

Original language	English
Journal	Pathogens
Early online date	8 Apr 2020
DOIs	https://doi.org/10.3390/pathogens9040271
Publication status	E-pub ahead of print - 8 Apr 2020

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pathogens-09-00271 (1)
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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Center for Tropical Livestock Genetics and Health: Genomic Tools to Improve the Productivity of Tropical Livestock and Increase Climatic Resilience, Adaptation, and Mitigation for the Benefit of Smallholder Farmers in Sub-Saharan Africa (DFID-DF)
Djikeng, A. (Principal Investigator)
Non-EU-based charities
15/11/17 → 31/03/22
Project: Research

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Dinga, J. N., Perimbie , S. N., Gamua, S. D., Chuma, F. N. G., Njimoh, D. L., Djikeng, A., Pelle, R., & Titanji, V. P. K. (2020). Analysis of the Role of TpUB05 Antigen from Theileria parva in Immune Responses to Malaria in Humans Compared to Its Homologue in Plasmodium falciparum the UB05 Antigen. Pathogens. Advance online publication. https://doi.org/10.3390/pathogens9040271

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title = "Analysis of the Role of TpUB05 Antigen from Theileria parva in Immune Responses to Malaria in Humans Compared to Its Homologue in Plasmodium falciparum the UB05 Antigen",

abstract = "Despite the amount of resources deployed and the technological advancements in molecular biology, vaccinology, immunology, genetics, and biotechnology, there are still no effective vaccines against malaria. Immunity to malaria is usually seen to be species- and/or strain-specific. However, there is a growing body of evidence suggesting the possibility of the existence of cross-strain, cross-species, and cross-genus immune responses in apicomplexans. The principle of gene conservation indicates that homologues play a similar role in closely related organisms. The homologue of UB05 in Theileria parva is TpUB05 (XP_763711.1), which has been tested and shown to be associated with protective immunity in East Coast fever. In a bid to identify potent markers of protective immunity to aid malaria vaccine development, TpUB05 was tested in malaria caused by Plasmodium falciparum. It was observed that TpUB05 was better at detecting antigen-specific antibodies in plasma compared to UB05 when tested by ELISA. The total IgG raised against TpUB05 was able to block parasitic growth in vitro more effectively than that raised against UB05. However, there was no significant difference between the two study antigens in recalling peripheral blood mononuclear cell (PBMC) memory through IFN-γ production. This study suggests, for the first time, that TpUB05 from T. parva cross-reacts with UB05 from P. falciparum and is a marker of protective immunity in malaria. Hence, TpUB05 should be considered for possible development as a potential subunit vaccine candidate against malaria",

author = "Dinga, {Jerome Nyhalah} and Perimbie, {Stephanie Numenyi} and Gamua, {Stanley Dobgima} and Chuma, {Francis N. G.} and Njimoh, {Dieudonne Lemuh} and Appolinaire Djikeng and Roger Pelle and Titanji, {Vincent Pryde Kehdingha}",

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AU - Dinga, Jerome Nyhalah

AU - Perimbie , Stephanie Numenyi

AU - Gamua, Stanley Dobgima

AU - Chuma, Francis N. G.

AU - Njimoh, Dieudonne Lemuh

AU - Djikeng, Appolinaire

AU - Pelle, Roger

AU - Titanji, Vincent Pryde Kehdingha

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AB - Despite the amount of resources deployed and the technological advancements in molecular biology, vaccinology, immunology, genetics, and biotechnology, there are still no effective vaccines against malaria. Immunity to malaria is usually seen to be species- and/or strain-specific. However, there is a growing body of evidence suggesting the possibility of the existence of cross-strain, cross-species, and cross-genus immune responses in apicomplexans. The principle of gene conservation indicates that homologues play a similar role in closely related organisms. The homologue of UB05 in Theileria parva is TpUB05 (XP_763711.1), which has been tested and shown to be associated with protective immunity in East Coast fever. In a bid to identify potent markers of protective immunity to aid malaria vaccine development, TpUB05 was tested in malaria caused by Plasmodium falciparum. It was observed that TpUB05 was better at detecting antigen-specific antibodies in plasma compared to UB05 when tested by ELISA. The total IgG raised against TpUB05 was able to block parasitic growth in vitro more effectively than that raised against UB05. However, there was no significant difference between the two study antigens in recalling peripheral blood mononuclear cell (PBMC) memory through IFN-γ production. This study suggests, for the first time, that TpUB05 from T. parva cross-reacts with UB05 from P. falciparum and is a marker of protective immunity in malaria. Hence, TpUB05 should be considered for possible development as a potential subunit vaccine candidate against malaria

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Analysis of the Role of TpUB05 Antigen from Theileria parva in Immune Responses to Malaria in Humans Compared to Its Homologue in Plasmodium falciparum the UB05 Antigen

Abstract

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Center for Tropical Livestock Genetics and Health: Genomic Tools to Improve the Productivity of Tropical Livestock and Increase Climatic Resilience, Adaptation, and Mitigation for the Benefit of Smallholder Farmers in Sub-Saharan Africa (DFID-DF)

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