Projects per year
Abstract / Description of output
Definitive haematopoietic stem cells (HSCs) first emerge in the aorta-gonad-mesonephros (AGM) region in both mice and human. An ex vivo culture approach has enabled recapitulation and analysis of murine HSC development. Knowledge of early human HSC development is hampered by scarcity of tissue: analysis of both CFU-C and HSC development in the human embryo is limited. Here we characterised the spatial distribution and temporal kinetics of CFU-C development within early human embryonic tissues. We then sought to adapt the murine ex vivo culture system to recapitulate human HSC development. We show robust expansion of CFU-Cs and maintenance, but no significant expansion, of human HSCs in culture. Furthermore, we demonstrate that HSCs emerge predominantly in the middle section of the dorsal aorta in our culture system. We conclude that there are important differences between early mouse and human haematopoiesis, which currently hinder the quest to recapitulate human HSC development ex vivo.
Original language | English |
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Pages (from-to) | 1056-1068 |
Number of pages | 13 |
Journal | Stem Cell Reports |
Volume | 12 |
Early online date | 4 Apr 2019 |
DOIs | |
Publication status | Published - 14 May 2019 |
Keywords / Materials (for Non-textual outputs)
- fetal liver
- hematopoietic progenitors
- hematopoietic stem cells
- human embryo
- yolk sac
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Dive into the research topics of 'Analysis of the spatiotemporal development of hematopoietic stem and progenitor cells in the early human embryo'. Together they form a unique fingerprint.Projects
- 3 Finished
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ECAT (100468) Analysis of the early development of human haematopoietic stem and progenitor cells
6/05/13 → 8/10/17
Project: Research
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Development of a non-invasive strategy enhancing haematopoietic stem cell potential of human umbilical cord cells
1/07/11 → 30/04/15
Project: Research
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Development of a non-invasive strategy enhancing haematopoietic stem cell potential of human umbilical cord cells
Turner, M.
1/07/11 → 30/04/15
Project: Research
Profiles
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Alexander Medvinsky
- School of Biological Sciences - Professor of Haematopoietic Stem Cell Biology
- Centre for Regenerative Medicine
- Edinburgh Haematopoiesis Network
Person: Academic: Research Active