Analyzing the Colocalization of MAP1LC3 and Lysosomal Markers in Primary Cells

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Abstract

This technique evaluates the colocalization of the autophagy protein MAP1LC3 (microtubule-associated proteins 1A/1B light chain 3B, here referred to as LC3) with lysosomes (autolysosomes) in primary cells in a high-throughput manner. It uses an imaging fluorescence-activated cell sorting cytometer called the ImageStream to concomitantly detect surface molecules, making possible the identification of cells in mixed cell populations (e.g., in blood or bone marrow). It can be applied to clinical samples and to rare cell populations because only a few cells are needed for detection.

Original languageEnglish
Pages (from-to)pdb.prot086272
JournalCold Spring Harbor protocols
Volume2015
Issue number9
DOIs
Publication statusPublished - 2015

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