Angiotensin-converting enzyme (CD143) marks hematopoietic stem cells in human embryonic, fetal, and adult hematopoietic tissues

Vanta J. Jokubaitis, Lidia Sinka, Rebecca Driessen, Genevieve Whitty, David N. Haylock, Ivan Bertoncello, Ian Smith, Bruno Peault, Manuela Tavian, Paul J. Simmons

Research output: Contribution to journalArticlepeer-review

Abstract

Previous studies revealed that mAb BB9 reacts with a subset of CD34(+) human BM cells with hematopoietic stem cell (HSC) characteristics. Here we map B89 expression throughout hernatopoietic development and show that the earliest definitive HSCs that arise at the ventral wall of the aorta and surrounding endothelial cells are BB9(+). Thereafter, BB9 is expressed by primitive hernatopoietic cells in fetal liver and in umbilical cord blood (UCB). BB9(+)CD34(+) UCB cells transplanted into nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice contribute 10-fold higher numbers of multilineage blood cells than their CD34(+)BB9(-) counterparts and contain a significantly higher incidence of SCID-repopulating cells than the unfractionated CD34(+) population. Protein microsequencing of the 160-kDa band corresponding to the BB9 protein established its identity as that of somatic anglotensin-converting enzyme (ACE). Although the role of ACE on human HSCs remains to be determined, these studies designate ACE as a hitherto unrecognized marker of human HSCs throughout hematopoietic ontogeny and adulthood.

Original languageEnglish
Pages (from-to)4055-4063
Number of pages9
JournalBlood
Volume111
Issue number8
Early online date9 Nov 2007
DOIs
Publication statusPublished - 15 Apr 2008

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