Antagonism of microRNA Function in Zebrafish Embryos by Using Locked Nucleic Acid Enzymes (LNAzymes)

Hemant Suryawanshi; Mukesh Kumar Lalwani; Soundhar Ramasamy; Rajiv Rana; Vinod Scaria; Sridhar Sivasubbu; Souvik Maiti

doi:10.1002/cbic.201100789

Antagonism of microRNA Function in Zebrafish Embryos by Using Locked Nucleic Acid Enzymes (LNAzymes)

Hemant Suryawanshi, Mukesh Kumar Lalwani, Soundhar Ramasamy, Rajiv Rana, Vinod Scaria, Sridhar Sivasubbu^*, Souvik Maiti^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

MicroRNAs (miRNAs) have crucial functions in many cellular processes, such as differentiation, proliferation and apoptosis; aberrant expression of miRNAs has been linked to human diseases, including cancer. Tools that allow specific and efficient knockdown of miRNAs would be of immense importance for exploring miRNA function. Zebrafish serves as an excellent vertebrate model system to understand the functions of miRNAs involved in a variety of biological processes. We designed and employed a strategy based on locked nucleic acid enzymes (LNAzymes) for in vivo knockdown of miRNA in zebrafish embryos. We demonstrate that LNAzyme can efficiently knockdown miRNAs with minimal toxicity to the zebrafish embryos.

Original language	English
Pages (from-to)	584-589
Number of pages	6
Journal	ChemBioChem
Volume	13
Issue number	4
Early online date	7 Feb 2012
DOIs	https://doi.org/10.1002/cbic.201100789
Publication status	Published - 5 Mar 2012

Keywords / Materials (for Non-textual outputs)

Enzymes
Locked nucleic acids
MicroRNA
Morpholino
Zebrafish

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10.1002/cbic.201100789

Cite this

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title = "Antagonism of microRNA Function in Zebrafish Embryos by Using Locked Nucleic Acid Enzymes (LNAzymes)",

abstract = "MicroRNAs (miRNAs) have crucial functions in many cellular processes, such as differentiation, proliferation and apoptosis; aberrant expression of miRNAs has been linked to human diseases, including cancer. Tools that allow specific and efficient knockdown of miRNAs would be of immense importance for exploring miRNA function. Zebrafish serves as an excellent vertebrate model system to understand the functions of miRNAs involved in a variety of biological processes. We designed and employed a strategy based on locked nucleic acid enzymes (LNAzymes) for in vivo knockdown of miRNA in zebrafish embryos. We demonstrate that LNAzyme can efficiently knockdown miRNAs with minimal toxicity to the zebrafish embryos.",

keywords = "Enzymes, Locked nucleic acids, MicroRNA, Morpholino, Zebrafish",

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year = "2012",

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doi = "10.1002/cbic.201100789",

language = "English",

volume = "13",

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journal = "ChemBioChem",

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T1 - Antagonism of microRNA Function in Zebrafish Embryos by Using Locked Nucleic Acid Enzymes (LNAzymes)

AU - Suryawanshi, Hemant

AU - Lalwani, Mukesh Kumar

AU - Ramasamy, Soundhar

AU - Rana, Rajiv

AU - Scaria, Vinod

AU - Sivasubbu, Sridhar

AU - Maiti, Souvik

PY - 2012/3/5

Y1 - 2012/3/5

N2 - MicroRNAs (miRNAs) have crucial functions in many cellular processes, such as differentiation, proliferation and apoptosis; aberrant expression of miRNAs has been linked to human diseases, including cancer. Tools that allow specific and efficient knockdown of miRNAs would be of immense importance for exploring miRNA function. Zebrafish serves as an excellent vertebrate model system to understand the functions of miRNAs involved in a variety of biological processes. We designed and employed a strategy based on locked nucleic acid enzymes (LNAzymes) for in vivo knockdown of miRNA in zebrafish embryos. We demonstrate that LNAzyme can efficiently knockdown miRNAs with minimal toxicity to the zebrafish embryos.

AB - MicroRNAs (miRNAs) have crucial functions in many cellular processes, such as differentiation, proliferation and apoptosis; aberrant expression of miRNAs has been linked to human diseases, including cancer. Tools that allow specific and efficient knockdown of miRNAs would be of immense importance for exploring miRNA function. Zebrafish serves as an excellent vertebrate model system to understand the functions of miRNAs involved in a variety of biological processes. We designed and employed a strategy based on locked nucleic acid enzymes (LNAzymes) for in vivo knockdown of miRNA in zebrafish embryos. We demonstrate that LNAzyme can efficiently knockdown miRNAs with minimal toxicity to the zebrafish embryos.

KW - Enzymes

KW - Locked nucleic acids

KW - MicroRNA

KW - Morpholino

KW - Zebrafish

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U2 - 10.1002/cbic.201100789

DO - 10.1002/cbic.201100789

M3 - Article

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AN - SCOPUS:84857565282

SN - 1439-4227

VL - 13

SP - 584

EP - 589

JO - ChemBioChem

JF - ChemBioChem

IS - 4

ER -

Antagonism of microRNA Function in Zebrafish Embryos by Using Locked Nucleic Acid Enzymes (LNAzymes)

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Keywords / Materials (for Non-textual outputs)

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