Anti-inflammatory and proliferative responses in human and ovine ovarian surface epithelial cells

O Gubbay; W Guo; M T Rae; D Niven; A F Howie; A S McNeilly; L Xu; S G Hillier

doi:10.1530/rep.1.00272

Anti-inflammatory and proliferative responses in human and ovine ovarian surface epithelial cells

O Gubbay, W Guo, M T Rae, D Niven, A F Howie, A S McNeilly, L Xu, S G Hillier

Deanery of Clinical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The majority of ovarian cancers (>90%) are believed to derive from the ovarian surface epithelium (OSE); a single layer covering the entire surface of the ovary. At ovulation, the OSE cell layer undergoes an inflammatory response, involving cell death and growth, in order to overcome ovarian surface rupture. Abnormalities during these processes are believed to contribute to the development of tumours. Using primary cultures of OSE cells, we have compared anti-inflammatory and proliferative responses directly between human and ovine OSE cells to further establish the use of ovine OSE cells as a suitable model system for the study of human OSE cells. In order to compare effects of inflammatory stimulation, expression and activity of 11betahydroxysteroid dehydrogenase (11betaHSD) type 1 was measured in OSE cells in response to interleukin (IL)-1alpha. As previously identified in human OSE cells, treatment of ovine OSE cells with IL-1alpha stimulated a concomitant increase of 11betaHSD type 1 mRNA (31-fold; P 1.2-fold (P 1.2-fold (P 1.5-fold (P

Original language	English
Pages (from-to)	607-14
Number of pages	8
Journal	Reproduction
Volume	128
Issue number	5
DOIs	https://doi.org/10.1530/rep.1.00272
Publication status	Published - 2004

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title = "Anti-inflammatory and proliferative responses in human and ovine ovarian surface epithelial cells",

abstract = "The majority of ovarian cancers (>90%) are believed to derive from the ovarian surface epithelium (OSE); a single layer covering the entire surface of the ovary. At ovulation, the OSE cell layer undergoes an inflammatory response, involving cell death and growth, in order to overcome ovarian surface rupture. Abnormalities during these processes are believed to contribute to the development of tumours. Using primary cultures of OSE cells, we have compared anti-inflammatory and proliferative responses directly between human and ovine OSE cells to further establish the use of ovine OSE cells as a suitable model system for the study of human OSE cells. In order to compare effects of inflammatory stimulation, expression and activity of 11betahydroxysteroid dehydrogenase (11betaHSD) type 1 was measured in OSE cells in response to interleukin (IL)-1alpha. As previously identified in human OSE cells, treatment of ovine OSE cells with IL-1alpha stimulated a concomitant increase of 11betaHSD type 1 mRNA (31-fold; P 1.2-fold (P 1.2-fold (P 1.5-fold (P ",

author = "O Gubbay and W Guo and Rae, {M T} and D Niven and Howie, {A F} and McNeilly, {A S} and L Xu and Hillier, {S G}",

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T1 - Anti-inflammatory and proliferative responses in human and ovine ovarian surface epithelial cells

AU - Gubbay, O

AU - Guo, W

AU - Rae, M T

AU - Niven, D

AU - Howie, A F

AU - McNeilly, A S

AU - Xu, L

AU - Hillier, S G

PY - 2004

Y1 - 2004

N2 - The majority of ovarian cancers (>90%) are believed to derive from the ovarian surface epithelium (OSE); a single layer covering the entire surface of the ovary. At ovulation, the OSE cell layer undergoes an inflammatory response, involving cell death and growth, in order to overcome ovarian surface rupture. Abnormalities during these processes are believed to contribute to the development of tumours. Using primary cultures of OSE cells, we have compared anti-inflammatory and proliferative responses directly between human and ovine OSE cells to further establish the use of ovine OSE cells as a suitable model system for the study of human OSE cells. In order to compare effects of inflammatory stimulation, expression and activity of 11betahydroxysteroid dehydrogenase (11betaHSD) type 1 was measured in OSE cells in response to interleukin (IL)-1alpha. As previously identified in human OSE cells, treatment of ovine OSE cells with IL-1alpha stimulated a concomitant increase of 11betaHSD type 1 mRNA (31-fold; P 1.2-fold (P 1.2-fold (P 1.5-fold (P

AB - The majority of ovarian cancers (>90%) are believed to derive from the ovarian surface epithelium (OSE); a single layer covering the entire surface of the ovary. At ovulation, the OSE cell layer undergoes an inflammatory response, involving cell death and growth, in order to overcome ovarian surface rupture. Abnormalities during these processes are believed to contribute to the development of tumours. Using primary cultures of OSE cells, we have compared anti-inflammatory and proliferative responses directly between human and ovine OSE cells to further establish the use of ovine OSE cells as a suitable model system for the study of human OSE cells. In order to compare effects of inflammatory stimulation, expression and activity of 11betahydroxysteroid dehydrogenase (11betaHSD) type 1 was measured in OSE cells in response to interleukin (IL)-1alpha. As previously identified in human OSE cells, treatment of ovine OSE cells with IL-1alpha stimulated a concomitant increase of 11betaHSD type 1 mRNA (31-fold; P 1.2-fold (P 1.2-fold (P 1.5-fold (P

U2 - 10.1530/rep.1.00272

DO - 10.1530/rep.1.00272

M3 - Article

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SN - 1470-1626

VL - 128

SP - 607

EP - 614

JO - Reproduction

JF - Reproduction

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ER -

Anti-inflammatory and proliferative responses in human and ovine ovarian surface epithelial cells

Abstract

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