Anti-parasitic benzoxaboroles are ineffective against Theileria parva in vitro

Pete Steketee, Edith Paxton, Michael P. Barrett, Michael C Pearce, Tim Connelley, Liam Morrison*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

East Coast Fever (ECF) is a disease affecting cattle in sub-Saharan Africa, caused by the tick borne Apicomplexan pathogen Theileria parva. The disease is a major problem for cattle farmers in affected regions and there are few methods of control, including a complex infection and treatment vaccine, expensive chemotherapy, and the more widespread tick control through acaricides. New intervention strategies are, therefore, sorely needed. Benzoxaboroles are a versatile class of boron-heterocyclic compounds with demonstrable pharmacological activity against a diverse group of pathogens, including those related to T. parva. In this study, the in vitro efficacy of three benzoxaboroles against the intracellular schizont stage of T. parva was investigated using a flow cytometry approach. Of the benzoxaboroles tested, only one showed any potency, albeit only at high concentrations, even though there is high protein sequence similarity in the CPSF3 protein target compared to other protozoan pathogen species. This finding suggests that benzoxaboroles currently of interest for the treatment of African animal trypanosomiasis, toxoplasmosis, cryptosporidiosis and malaria may not be suitable for the treatment of ECF. We conclude that testing of further benzoxaborole compounds is needed to fully determine whether any lead compounds can be identified to target T. parva.
Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalInternational Journal for Parasitology: Drugs and Drug Resistance
Early online date10 Oct 2023
Publication statusPublished - Dec 2023


Dive into the research topics of 'Anti-parasitic benzoxaboroles are ineffective against Theileria parva in vitro'. Together they form a unique fingerprint.

Cite this