Antibody and cytokine responses in efferent lymph following vaccination with different adjuvants

J S Rothel, L A Corner, MW Lightowlers, H-F Seow, P McWaters, Gary Entrican, P. R. Wood

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The cannulated efferent lymph node in sheep was used to examine the effect of different adjuvants on the antibody and cytokine responses following sub-cutaneous vaccination with a recombinant Taenia ovis antigen (45 W). Vaccination with Quil A elicited relatively higher levels of IgM than did IFA or Al(OH)3. In general, 45 W specific IgG1 and IgG2 titres were higher and maintained for longer periods of time in lymph from sheep vaccinated with IFA and lower and shorter lived in animals which received the Al(OH)3 based vaccine. Interferon-gamma was present within one day in efferent lymph from all sheep which received the Quil A formulation and in only one of the three sheep that received the IFA formulation. GM-CSF was only detected in lymph from sheep vaccinated with the IFA formulation. IL-8 was present in lymph prior to vaccination and only animals which received the Quil A formulation had increased levels of IL-8 after vaccination. Neither of the inflammatory cytokines IL-1 beta and TNF alpha were detected in efferent lymph from any animals in this study. This paper highlights the potential of the lymphatic cannulation model for investigations of the in vivo action of adjuvants.
Original languageEnglish
Pages (from-to)167-83
JournalVeterinary Immunology and Immunopathology
Volume63
Issue number1-2
DOIs
Publication statusPublished - 15 May 1998

Keywords / Materials (for Non-textual outputs)

  • Adjuvants, Immunologic
  • Animals
  • Antibodies, Helminth
  • Antigens, Helminth
  • Catheterization
  • Cytokines
  • Enzyme-Linked Immunosorbent Assay
  • Helminth Proteins
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Interleukin-8
  • Lymph
  • Recombiant proteins
  • saponins
  • Sheep
  • Taenia
  • Vaccination

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