Antigenicity of recombinant proteins derived from Plasmodium falciparum merozoite surface protein 1

D R Cavanagh, J S McBride, David Cavanagh

Research output: Contribution to journalArticlepeer-review

Abstract

We have expressed seven recombinant antigens representing two N-terminal regions of the polymorphic merozoite surface protein 1 (MSP-1) of Plasmodium falciparum. The antigens include the MAD20 and Palo Alto forms of the relatively conserved Block 1 region, and variants of the Block 2 region from isolates 3D7, Palo Alto FUP, MAD20, Wellcome and RO33, that are representative of a range of amino acid sequence diversity in this most polymorphic section of MSP-1. All recombinant antigens have been able to immunise mice to produce polyclonal antibodies which specifically recognise parasite MSP-1 in indirect immunofluorescence assays and in Western blots. The recombinant antigens also react appropriately in ELISA with murine monoclonal antibodies specific for variant epitopes in Block 2 of MSP-1. These results show that the antigenic structure of the recombinant proteins is similar to that of the native MSP-1 product from parasites. Importantly, human sera from malaria-exposed individuals contain IgG antibodies that recognise very specifically one or another of the Block 2 types, showing that different Block 2 types are immunogenic, antigenically distinct and distinguishable when presented during natural infections. In contrast, the conserved Block 1 is rarely recognised by human antibodies. (C) 1997 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)197-211
Number of pages15
JournalMolecular and Biochemical Parasitology
Volume85
Issue number2
Publication statusPublished - Apr 1997

Keywords

  • malaria
  • Plasmodium falciparum
  • antibody
  • recombinant antigens
  • HUMAN MALARIA PARASITES
  • ESCHERICHIA-COLI
  • AOTUS MONKEYS
  • IMMUNE-RESPONSE
  • PRECURSOR
  • GENE
  • GLYCOPROTEIN
  • POLYMORPHISM
  • ANTIBODIES
  • POPULATION

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