APC/C(Cdh1) Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity

Ahmed Rattani; Randy Ballesteros Mejia; Katherine Roberts; Maurici B Roig; Jonathan Godwin; Michael Hopkins; Manuel Eguren; Luis Sanchez-Pulido; Elwy Okaz; Sugako Ogushi; Magda Wolna; Jean Metson; Alberto M Pendás; Marcos Malumbres; Béla Novák; Mary Herbert; Kim Nasmyth

doi:10.1016/j.cub.2017.04.023

APC/C(Cdh1) Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity

Ahmed Rattani, Randy Ballesteros Mejia, Katherine Roberts, Maurici B Roig, Jonathan Godwin, Michael Hopkins, Manuel Eguren, Luis Sanchez-Pulido, Elwy Okaz, Sugako Ogushi, Magda Wolna, Jean Metson, Alberto M Pendás, Marcos Malumbres, Béla Novák, Mary Herbert, Kim Nasmyth

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Abstract / Description of output

In mammalian females, germ cells remain arrested as primordial follicles. Resumption of meiosis is heralded by germinal vesicle breakdown, condensation of chromosomes, and their eventual alignment on metaphase plates. At the first meiotic division, anaphase-promoting complex/cyclosome associated with Cdc20 (APC/C(Cdc20)) activates separase and thereby destroys cohesion along chromosome arms. Because cohesion around centromeres is protected by shugoshin-2, sister chromatids remain attached through centromeric/pericentromeric cohesin. We show here that, by promoting proteolysis of cyclins and Cdc25B at the germinal vesicle (GV) stage, APC/C associated with the Cdh1 protein (APC/C(Cdh1)) delays the increase in Cdk1 activity, leading to germinal vesicle breakdown (GVBD). More surprisingly, by moderating the rate at which Cdk1 is activated following GVBD, APC/C(Cdh1) creates conditions necessary for the removal of shugoshin-2 from chromosome arms by the Aurora B/C kinase, an event crucial for the efficient resolution of chiasmata.

Original language	English
Pages (from-to)	1462-1476.e5
Journal	Current biology : CB
Volume	27
Issue number	10
Early online date	11 May 2017
DOIs	https://doi.org/10.1016/j.cub.2017.04.023
Publication status	Published - 22 May 2017

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Journal Article

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10.1016/j.cub.2017.04.023

1-s2.0-S0960982217304323-main
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Rattani, A., Ballesteros Mejia, R., Roberts, K., Roig, M. B., Godwin, J., Hopkins, M., Eguren, M., Sanchez-Pulido, L., Okaz, E., Ogushi, S., Wolna, M., Metson, J., Pendás, A. M., Malumbres, M., Novák, B., Herbert, M., & Nasmyth, K. (2017). APC/C(Cdh1) Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity. Current biology : CB, 27(10), 1462-1476.e5. https://doi.org/10.1016/j.cub.2017.04.023

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title = "APC/C(Cdh1) Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity",

abstract = "In mammalian females, germ cells remain arrested as primordial follicles. Resumption of meiosis is heralded by germinal vesicle breakdown, condensation of chromosomes, and their eventual alignment on metaphase plates. At the first meiotic division, anaphase-promoting complex/cyclosome associated with Cdc20 (APC/C(Cdc20)) activates separase and thereby destroys cohesion along chromosome arms. Because cohesion around centromeres is protected by shugoshin-2, sister chromatids remain attached through centromeric/pericentromeric cohesin. We show here that, by promoting proteolysis of cyclins and Cdc25B at the germinal vesicle (GV) stage, APC/C associated with the Cdh1 protein (APC/C(Cdh1)) delays the increase in Cdk1 activity, leading to germinal vesicle breakdown (GVBD). More surprisingly, by moderating the rate at which Cdk1 is activated following GVBD, APC/C(Cdh1) creates conditions necessary for the removal of shugoshin-2 from chromosome arms by the Aurora B/C kinase, an event crucial for the efficient resolution of chiasmata.",

keywords = "Journal Article",

author = "Ahmed Rattani and {Ballesteros Mejia}, Randy and Katherine Roberts and Roig, {Maurici B} and Jonathan Godwin and Michael Hopkins and Manuel Eguren and Luis Sanchez-Pulido and Elwy Okaz and Sugako Ogushi and Magda Wolna and Jean Metson and Pend{\'a}s, {Alberto M} and Marcos Malumbres and B{\'e}la Nov{\'a}k and Mary Herbert and Kim Nasmyth",

year = "2017",

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doi = "10.1016/j.cub.2017.04.023",

language = "English",

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Rattani, A, Ballesteros Mejia, R, Roberts, K, Roig, MB, Godwin, J, Hopkins, M, Eguren, M, Sanchez-Pulido, L, Okaz, E, Ogushi, S, Wolna, M, Metson, J, Pendás, AM, Malumbres, M, Novák, B, Herbert, M & Nasmyth, K 2017, 'APC/C(Cdh1) Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity', Current biology : CB, vol. 27, no. 10, pp. 1462-1476.e5. https://doi.org/10.1016/j.cub.2017.04.023

TY - JOUR

T1 - APC/C(Cdh1) Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity

AU - Rattani, Ahmed

AU - Ballesteros Mejia, Randy

AU - Roberts, Katherine

AU - Roig, Maurici B

AU - Godwin, Jonathan

AU - Hopkins, Michael

AU - Eguren, Manuel

AU - Sanchez-Pulido, Luis

AU - Okaz, Elwy

AU - Ogushi, Sugako

AU - Wolna, Magda

AU - Metson, Jean

AU - Pendás, Alberto M

AU - Malumbres, Marcos

AU - Novák, Béla

AU - Herbert, Mary

AU - Nasmyth, Kim

PY - 2017/5/22

Y1 - 2017/5/22

N2 - In mammalian females, germ cells remain arrested as primordial follicles. Resumption of meiosis is heralded by germinal vesicle breakdown, condensation of chromosomes, and their eventual alignment on metaphase plates. At the first meiotic division, anaphase-promoting complex/cyclosome associated with Cdc20 (APC/C(Cdc20)) activates separase and thereby destroys cohesion along chromosome arms. Because cohesion around centromeres is protected by shugoshin-2, sister chromatids remain attached through centromeric/pericentromeric cohesin. We show here that, by promoting proteolysis of cyclins and Cdc25B at the germinal vesicle (GV) stage, APC/C associated with the Cdh1 protein (APC/C(Cdh1)) delays the increase in Cdk1 activity, leading to germinal vesicle breakdown (GVBD). More surprisingly, by moderating the rate at which Cdk1 is activated following GVBD, APC/C(Cdh1) creates conditions necessary for the removal of shugoshin-2 from chromosome arms by the Aurora B/C kinase, an event crucial for the efficient resolution of chiasmata.

AB - In mammalian females, germ cells remain arrested as primordial follicles. Resumption of meiosis is heralded by germinal vesicle breakdown, condensation of chromosomes, and their eventual alignment on metaphase plates. At the first meiotic division, anaphase-promoting complex/cyclosome associated with Cdc20 (APC/C(Cdc20)) activates separase and thereby destroys cohesion along chromosome arms. Because cohesion around centromeres is protected by shugoshin-2, sister chromatids remain attached through centromeric/pericentromeric cohesin. We show here that, by promoting proteolysis of cyclins and Cdc25B at the germinal vesicle (GV) stage, APC/C associated with the Cdh1 protein (APC/C(Cdh1)) delays the increase in Cdk1 activity, leading to germinal vesicle breakdown (GVBD). More surprisingly, by moderating the rate at which Cdk1 is activated following GVBD, APC/C(Cdh1) creates conditions necessary for the removal of shugoshin-2 from chromosome arms by the Aurora B/C kinase, an event crucial for the efficient resolution of chiasmata.

KW - Journal Article

U2 - 10.1016/j.cub.2017.04.023

DO - 10.1016/j.cub.2017.04.023

M3 - Article

C2 - 28502659

SN - 0960-9822

VL - 27

SP - 1462-1476.e5

JO - Current biology : CB

JF - Current biology : CB

IS - 10

ER -

APC/C(Cdh1) Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity

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