Application of epifluorescence scanning for monitoring the efficacy of protein removal by RF gas-plasma decontamination

Helen C. Baxter; Patricia R. Richardson; Gaynor A. Campbell; Valeri I. Kovalev; Robert Maier; James S. Barton; Anita C. Jones; Greg DeLarge; Mark Casey; Robert L. Baxter

doi:10.1088/1367-2630/11/11/115028

Application of epifluorescence scanning for monitoring the efficacy of protein removal by RF gas-plasma decontamination

Helen C. Baxter, Patricia R. Richardson, Gaynor A. Campbell, Valeri I. Kovalev, Robert Maier, James S. Barton, Anita C. Jones, Greg DeLarge, Mark Casey, Robert L. Baxter

Research output: Contribution to journal › Article › peer-review

Abstract

The development of methods for measuring the efficiency of gas-plasma decontamination has lagged far behind application. An approach to measuring the efficiency of protein removal from solid surfaces using fluorescein-labelled bovine serum albumin and epifluorescence scanning (EFSCAN) is described. A method for fluorescently labelling proteins, which are adsorbed and denatured on metal surfaces, has been developed. Both approaches have been used to evaluate the efficiency of radio frequency (RF) gas-plasma decontamination protocols. Examples with 'real' surgical instruments demonstrate that an argon-oxygen RF gas-plasma treatment can routinely reduce the protein load by about three orders of magnitude beyond that achieved by current decontamination methods.

Original language	English
Article number	115028
Pages (from-to)	-
Number of pages	13
Journal	New Journal of Physics
Volume	11
DOIs	https://doi.org/10.1088/1367-2630/11/11/115028
Publication status	Published - 26 Nov 2009

Keywords / Materials (for Non-textual outputs)

SURGICAL-INSTRUMENTS
SURFACE
CONTAMINATION
SPECTROSCOPY
DISCHARGES

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10.1088/1367-2630/11/11/115028

Cite this

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title = "Application of epifluorescence scanning for monitoring the efficacy of protein removal by RF gas-plasma decontamination",

abstract = "The development of methods for measuring the efficiency of gas-plasma decontamination has lagged far behind application. An approach to measuring the efficiency of protein removal from solid surfaces using fluorescein-labelled bovine serum albumin and epifluorescence scanning (EFSCAN) is described. A method for fluorescently labelling proteins, which are adsorbed and denatured on metal surfaces, has been developed. Both approaches have been used to evaluate the efficiency of radio frequency (RF) gas-plasma decontamination protocols. Examples with 'real' surgical instruments demonstrate that an argon-oxygen RF gas-plasma treatment can routinely reduce the protein load by about three orders of magnitude beyond that achieved by current decontamination methods.",

keywords = "SURGICAL-INSTRUMENTS, SURFACE, CONTAMINATION, SPECTROSCOPY, DISCHARGES",

author = "Baxter, {Helen C.} and Richardson, {Patricia R.} and Campbell, {Gaynor A.} and Kovalev, {Valeri I.} and Robert Maier and Barton, {James S.} and Jones, {Anita C.} and Greg DeLarge and Mark Casey and Baxter, {Robert L.}",

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AU - Baxter, Helen C.

AU - Richardson, Patricia R.

AU - Campbell, Gaynor A.

AU - Kovalev, Valeri I.

AU - Maier, Robert

AU - Barton, James S.

AU - Jones, Anita C.

AU - DeLarge, Greg

AU - Casey, Mark

AU - Baxter, Robert L.

PY - 2009/11/26

Y1 - 2009/11/26

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AB - The development of methods for measuring the efficiency of gas-plasma decontamination has lagged far behind application. An approach to measuring the efficiency of protein removal from solid surfaces using fluorescein-labelled bovine serum albumin and epifluorescence scanning (EFSCAN) is described. A method for fluorescently labelling proteins, which are adsorbed and denatured on metal surfaces, has been developed. Both approaches have been used to evaluate the efficiency of radio frequency (RF) gas-plasma decontamination protocols. Examples with 'real' surgical instruments demonstrate that an argon-oxygen RF gas-plasma treatment can routinely reduce the protein load by about three orders of magnitude beyond that achieved by current decontamination methods.

KW - SURGICAL-INSTRUMENTS

KW - SURFACE

KW - CONTAMINATION

KW - SPECTROSCOPY

KW - DISCHARGES

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Application of epifluorescence scanning for monitoring the efficacy of protein removal by RF gas-plasma decontamination

Abstract

Keywords / Materials (for Non-textual outputs)

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