Application of molecular techniques in the rapid diagnosis of EBV-associated oral hairy leukoplakia

H A Cubie; D H Felix; J C Southam; D Wray

Application of molecular techniques in the rapid diagnosis of EBV-associated oral hairy leukoplakia

H A Cubie, D H Felix, J C Southam, D Wray

College of Medicine and Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

A rapid method for the detection of EBV-DNA in paraffin sections of lesions of oral hairy leukoplakia (OHL) is described. The method makes use of advances in molecular technology, including the use of synthetic oligonucleotides with digoxigenin labelling in an in situ hybridisation (ISH) reaction, which can be completed in 24 h. Using this method, sections from 15 of 17 patients clinically diagnosed as having OHL contained readily detectable EBV-DNA in small foci along the upper layers of the stratum spinosum. The sections examined from the two remaining patients appeared to be EBV-DNA negative but both patients were on AZT therapy and one was in addition receiving acyclovir.

Original language	English
Pages (from-to)	271-4
Number of pages	4
Journal	Journal of Oral Pathology and Medicine
Volume	20
Issue number	6
Publication status	Published - Jul 1991

Keywords

Acquired Immunodeficiency Syndrome
Cell Nucleus
DNA Probes
DNA, Viral
HIV Seropositivity
Herpesvirus 4, Human
Humans
Leukoplakia, Oral
Nucleic Acid Hybridization
Staining and Labeling
Tongue Neoplasms
Tumor Virus Infections

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title = "Application of molecular techniques in the rapid diagnosis of EBV-associated oral hairy leukoplakia",

abstract = "A rapid method for the detection of EBV-DNA in paraffin sections of lesions of oral hairy leukoplakia (OHL) is described. The method makes use of advances in molecular technology, including the use of synthetic oligonucleotides with digoxigenin labelling in an in situ hybridisation (ISH) reaction, which can be completed in 24 h. Using this method, sections from 15 of 17 patients clinically diagnosed as having OHL contained readily detectable EBV-DNA in small foci along the upper layers of the stratum spinosum. The sections examined from the two remaining patients appeared to be EBV-DNA negative but both patients were on AZT therapy and one was in addition receiving acyclovir.",

keywords = "Acquired Immunodeficiency Syndrome, Cell Nucleus, DNA Probes, DNA, Viral, HIV Seropositivity, Herpesvirus 4, Human, Humans, Leukoplakia, Oral, Nucleic Acid Hybridization, Staining and Labeling, Tongue Neoplasms, Tumor Virus Infections",

author = "Cubie, {H A} and Felix, {D H} and Southam, {J C} and D Wray",

year = "1991",

month = jul,

language = "English",

volume = "20",

pages = "271--4",

journal = "Journal of Oral Pathology and Medicine",

issn = "0904-2512",

publisher = "Wiley",

number = "6",

}

TY - JOUR

T1 - Application of molecular techniques in the rapid diagnosis of EBV-associated oral hairy leukoplakia

AU - Cubie, H A

AU - Felix, D H

AU - Southam, J C

AU - Wray, D

PY - 1991/7

Y1 - 1991/7

N2 - A rapid method for the detection of EBV-DNA in paraffin sections of lesions of oral hairy leukoplakia (OHL) is described. The method makes use of advances in molecular technology, including the use of synthetic oligonucleotides with digoxigenin labelling in an in situ hybridisation (ISH) reaction, which can be completed in 24 h. Using this method, sections from 15 of 17 patients clinically diagnosed as having OHL contained readily detectable EBV-DNA in small foci along the upper layers of the stratum spinosum. The sections examined from the two remaining patients appeared to be EBV-DNA negative but both patients were on AZT therapy and one was in addition receiving acyclovir.

AB - A rapid method for the detection of EBV-DNA in paraffin sections of lesions of oral hairy leukoplakia (OHL) is described. The method makes use of advances in molecular technology, including the use of synthetic oligonucleotides with digoxigenin labelling in an in situ hybridisation (ISH) reaction, which can be completed in 24 h. Using this method, sections from 15 of 17 patients clinically diagnosed as having OHL contained readily detectable EBV-DNA in small foci along the upper layers of the stratum spinosum. The sections examined from the two remaining patients appeared to be EBV-DNA negative but both patients were on AZT therapy and one was in addition receiving acyclovir.

KW - Acquired Immunodeficiency Syndrome

KW - Cell Nucleus

KW - DNA Probes

KW - DNA, Viral

KW - HIV Seropositivity

KW - Herpesvirus 4, Human

KW - Humans

KW - Leukoplakia, Oral

KW - Nucleic Acid Hybridization

KW - Staining and Labeling

KW - Tongue Neoplasms

KW - Tumor Virus Infections

M3 - Article

C2 - 1716319

VL - 20

SP - 271

EP - 274

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

SN - 0904-2512

IS - 6

ER -

Application of molecular techniques in the rapid diagnosis of EBV-associated oral hairy leukoplakia

Abstract

Keywords

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